Ono Y, Kurokawa T, Fujii T, Kawahara K, Igarashi K, Kikkawa U, Ogita K, Nishizuka Y
FEBS Lett. 1986 Oct 6;206(2):347-52. doi: 10.1016/0014-5793(86)81010-9.
Two types of complementary DNA clones for rat brain protein kinase C were isolated. These clones encode 671 and 673 amino acid sequences, which differ from each other only in the carboxyl-terminal regions of approx. 50 amino acid residues. This difference seems to result from alternative splicing. Elucidation of the sequences of these cDNA clones as well as some peptides from the purified rat brain enzyme suggests the existence of an additional species of protein kinase C in this tissue. It is attractive to imagine that the heterogeneity of protein kinase C may reflect diverse pathways of signal transduction into the cell.
分离出了两种大鼠脑蛋白激酶C的互补DNA克隆。这些克隆编码671和673个氨基酸序列，它们彼此之间仅在约50个氨基酸残基的羧基末端区域有所不同。这种差异似乎是由可变剪接导致的。对这些cDNA克隆以及纯化的大鼠脑酶中的一些肽段序列的阐明表明，该组织中存在另一种蛋白激酶C。可以想象，蛋白激酶C的异质性可能反映了细胞内信号转导的多种途径，这很有吸引力。
