Pujari Shiuli, Hoess Andreas, Shen Jinhui, Thormann Annika, Heilmann Andreas, Tang Liping, Karlsson-Ott Marjam
Applied Material Science, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, SE-751 21, Uppsala, Sweden.
J Biomed Mater Res A. 2014 Nov;102(11):3773-80. doi: 10.1002/jbm.a.35048. Epub 2013 Dec 9.
The present study focuses on the effects of nanoscale porosity on inflammatory response in vitro and in vivo. Nanoporous alumina membranes with different pore sizes, 20 and 200 nm in diameter, were used. We first evaluated cell/alumina interactions in vitro by observing adhesion, proliferation, and activation of a murine fibroblast and a macrophage cell line. To investigate the chronic inflammatory response, the membranes were implanted subcutaneously in mice for 2 weeks. Cell recruitment to the site of implantation was determined by histology and the production of cytokines was measured by protein array analysis. Both in vitro and in vivo studies showed that 200 nm pores induced a stronger inflammatory response as compared to the alumina with 20 nm pores. This was observed by an increase in macrophage activation in vitro as well as higher cell recruitment and generation of proinflammatory cytokines around the alumina with 200 nm pores, in vivo. Our results suggest that nanofeatures can be modulated in order to control the inflammatory response to implants.
本研究聚焦于纳米级孔隙率对体内外炎症反应的影响。使用了孔径分别为20纳米和200纳米的纳米多孔氧化铝膜。我们首先通过观察小鼠成纤维细胞和巨噬细胞系的黏附、增殖及活化情况，在体外评估细胞与氧化铝的相互作用。为研究慢性炎症反应，将这些膜皮下植入小鼠体内2周。通过组织学确定植入部位的细胞募集情况，并通过蛋白质阵列分析测量细胞因子的产生。体外和体内研究均表明，与孔径为20纳米的氧化铝相比，孔径为200纳米的孔隙引发了更强的炎症反应。这在体外表现为巨噬细胞活化增加，在体内则表现为孔径为200纳米的氧化铝周围有更高的细胞募集和促炎细胞因子生成。我们的结果表明，可以对纳米特征进行调节，以控制对植入物的炎症反应。
