MacFarlane T W, McCourtie J, Watkins T R
J Clin Periodontol. 1986 Aug;13(7):651-3. doi: 10.1111/j.1600-051x.1986.tb00860.x.
A new method for counting the numbers of spirochaetes in subgingival plaque is described. The technique involves the combined use of a negative stained preparation, dark field microscopy, and an image analysing system. Advantages of the method include: smears need not be made until 2 days after sampling, and can be examined microscopically for up to 1 year after preparation; reproducibility of counts are more than 90% and the data can be computerised. The new method should prove useful in the routine assessment of subgingival plaque from patients with various forms of periodontitis, and in epidemiological surveys.
本文描述了一种计数龈下菌斑中螺旋体数量的新方法。该技术包括负染色标本、暗视野显微镜和图像分析系统的联合使用。该方法的优点包括：采样后2天内无需制作涂片，制备后可在显微镜下检查长达1年；计数的重现性超过90%，数据可进行计算机处理。新方法在对各种形式牙周炎患者的龈下菌斑进行常规评估以及流行病学调查中应会证明有用。
