Coffey A, Coulter W A, Linden G J
Department of Microbiology, Queen's University, Belfast, N. Ireland.
J Periodontal Res. 1995 Sep;30(5):342-8. doi: 10.1111/j.1600-0765.1995.tb01285.x.
The purpose of the present study was to compare direct light microscopy using a silver stain, with dark field microscopy for differential counting of subgingival plaque samples from patients with periodontitis. The feasibility of using the staining method was assessed on subgingival plaque samples which were collected at 34 sites from patients with untreated adult periodontitis. Differential counts of plaque morphotypes assessed by both methods showed close agreement. The proportions of spirochaetes assessed by both methods were significantly associated with probing depth. The silver stain proved a simple, rapid and inexpensive method for differential counting of plaque composition. This method is suitable for possible use in general dental practice where smears could be made at the chair-side and allowed to dry ready for staining and counting at the dentist's convenience using a simple light microscope. A permanent record is also produced which could allow for comparison of samples from before and after treatment and could be used as an aid to patient motivation in suitable cases.
本研究的目的是比较使用银染的直接光学显微镜与暗视野显微镜，用于对牙周炎患者龈下菌斑样本进行鉴别计数。在从未经治疗的成人牙周炎患者的34个部位采集的龈下菌斑样本上评估了使用该染色方法的可行性。两种方法评估的菌斑形态类型鉴别计数显示出密切的一致性。两种方法评估的螺旋体比例与探诊深度显著相关。银染被证明是一种用于菌斑成分鉴别计数的简单、快速且廉价的方法。这种方法适用于一般牙科实践，在那里可以在椅旁制作涂片并晾干，以便在牙医方便的时候使用简单的光学显微镜进行染色和计数。还会生成一份永久记录，可用于比较治疗前后的样本，并在合适的情况下作为激励患者的辅助手段。
