Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Jeonbuk 570-749, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:475386. doi: 10.1155/2013/475386. Epub 2013 Oct 30.
Rubi Fructus (RF) is known to exert several pharmacological effects including antitumor, antioxidant, and anti-inflammatory activities. However, its antiobesity effect has not been reported yet. This study was focused on the antidifferentiation effect of RF extract on 3T3-L1 preadipocytes. When 3T3-L1 preadipocytes were differentiating into adipocytes, 10-100 μ g/mL of RF was added. Next, the lipid contents were quantified by Oil Red O staining. RF significantly reduced lipid accumulation and downregulated the expression of peroxisome proliferator-activated receptor γ (PPAR γ ), CCAAT0-enhancer-binding proteins α (C/EBP α ), adipocyte fatty acid-binding protein 2 (aP2), resistin, and adiponectin in ways that were concentration dependent. Moreover, RF markedly upregulated liver kinase B1 and AMP-activated protein kinase (AMPK). Interestingly, pretreatment with AMPK α siRNA and RF downregulated the expression of PPAR γ and C/EBP α protein as well as the adipocyte differentiation. Our study shows that RF is capable of inhibiting the differentiation of 3T3-L1 adipocytes through the modulation of PPAR γ , C/EBP α , and AMPK, suggesting that it has a potential for therapeutic application in the treatment or prevention of obesity.
枸杞（RF）具有多种药理作用，包括抗肿瘤、抗氧化和抗炎作用。然而，其抗肥胖作用尚未见报道。本研究主要集中在 RF 提取物对 3T3-L1 前脂肪细胞的抗分化作用上。当 3T3-L1 前脂肪细胞向脂肪细胞分化时，加入 10-100μg/mL 的 RF。然后，通过油红 O 染色定量脂质含量。RF 显著减少了脂质积累，并呈浓度依赖性地下调了过氧化物酶体增殖物激活受体 γ（PPAR γ）、CCAAT0 增强子结合蛋白 α（C/EBP α）、脂肪细胞脂肪酸结合蛋白 2（aP2）、抵抗素和脂联素的表达。此外，RF 明显上调了肝激酶 B1 和 AMP 激活的蛋白激酶（AMPK）。有趣的是，用 AMPK α siRNA 预处理和 RF 下调了 PPAR γ 和 C/EBP α 蛋白的表达以及脂肪细胞分化。我们的研究表明，RF 通过调节 PPAR γ、C/EBP α 和 AMPK 抑制 3T3-L1 脂肪细胞的分化，表明其在肥胖症的治疗或预防方面具有潜在的应用价值。
