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未成熟的悬钩子及其成分的抗肥胖作用：潜在机制的体外和体内表征

Antiobesity Effects of Unripe Rubus coreanus Miquel and Its Constituents: An In Vitro and In Vivo Characterization of the Underlying Mechanism.

作者信息

Oh Dool-Ri, Kim Yujin, Choi Eun-Jin, Jung Myung-A, Bae Donghyuck, Jo Ara, Kim Young Ran, Kim Sunoh

机构信息

Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR), Jeollanamdo 529-851, Republic of Korea; College of Pharmacy, Chonnam National University, Gwangju 500-757, Republic of Korea.

Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR), Jeollanamdo 529-851, Republic of Korea.

出版信息

Evid Based Complement Alternat Med. 2016;2016:4357656. doi: 10.1155/2016/4357656. Epub 2016 Jan 19.

DOI:10.1155/2016/4357656
PMID:26904142
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4745304/
Abstract

Background. The objective of the present study was to perform a bioguided fractionation of unripe Rubus coreanus Miquel (uRC) and evaluate the lipid accumulation system involvement in its antiobesity activity as well as study the uRC mechanism of action. Results. After the fractionation, the BuOH fraction of uRC (uRCB) was the most active fraction, suppressing the differentiation of 3T3-L1 adipocytes in a dose-dependent manner. Moreover, after an oral administration for 8 weeks in HFD-induced obese mice, uRCB (10 and 50 mg/kg/day) produced a significant decrease in body weight, food efficiency ratio, adipose tissue weight and LDL-cholesterol, serum glucose, TC, and TG levels. Similarly, uRCB significantly suppressed the elevated mRNA levels of PPARγ in the adipose tissue in vivo. Next, we investigated the antiobesity effects of ellagic acid, erycibelline, 5-hydroxy-2-pyridinemethanol, m-hydroxyphenylglycine, and 4-hydroxycoumarin isolated from uRCB. Without affecting cell viability, five bioactive compounds decreased the lipid accumulation in the 3T3-L1 cells and the mRNA expression levels of key adipogenic genes such as PPARγ, C/EBPα, SREBP-1c, ACC, and FAS. Conclusion. These results suggest that uRC and its five bioactive compounds may be a useful therapeutic agent for body weight control by downregulating adipogenesis and lipogenesis.

摘要

背景。本研究的目的是对未成熟的悬钩子（uRC）进行生物导向分级分离，评估其抗肥胖活性中脂质积累系统的参与情况，并研究uRC的作用机制。结果。分级分离后，uRC的正丁醇级分（uRCB）是活性最高的级分，以剂量依赖的方式抑制3T3-L1脂肪细胞的分化。此外，在高脂饮食诱导的肥胖小鼠中口服给药8周后，uRCB（10和50mg/kg/天）使体重、食物效率比、脂肪组织重量以及低密度脂蛋白胆固醇、血糖、总胆固醇和甘油三酯水平显著降低。同样，uRCB在体内显著抑制了脂肪组织中PPARγ mRNA水平的升高。接下来，我们研究了从uRCB中分离出的鞣花酸、刺囊酸、5-羟基-2-吡啶甲醇、间羟基苯甘氨酸和4-羟基香豆素的抗肥胖作用。在不影响细胞活力的情况下，五种生物活性化合物降低了3T3-L1细胞中的脂质积累以及关键脂肪生成基因如PPARγ、C/EBPα、SREBP-1c、ACC和FAS的mRNA表达水平。结论。这些结果表明，uRC及其五种生物活性化合物可能是通过下调脂肪生成和脂质生成来控制体重的有用治疗剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d2b/4745304/81a53ab74dce/ECAM2016-4357656.008.jpg
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2
6,6'-Bieckol inhibits adipocyte differentiation through downregulation of adipogenesis and lipogenesis in 3T3-L1 cells.6,6'-联表儿茶素通过下调3T3-L1细胞中的脂肪生成和脂质生成来抑制脂肪细胞分化。
J Sci Food Agric. 2015 Jul;95(9):1830-7. doi: 10.1002/jsfa.6881. Epub 2014 Oct 28.
3
Rubi Fructus (Rubus coreanus) activates the expression of thermogenic genes in vivo and in vitro.
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Nutrients. 2022 May 6;14(9):1951. doi: 10.3390/nu14091951.
4
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5
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J Agric Food Chem. 2012 Sep 12;60(36):9089-96. doi: 10.1021/jf3017404. Epub 2012 Aug 27.