Nam Daewoong, Park Jaehyun, Gallagher-Jones Marcus, Shimada Hiroki, Kim Sangsoo, Kim Sunam, Kohmura Yoshiki, Ishikawa Tetsuya, Song Changyong
RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
Rev Sci Instrum. 2013 Nov;84(11):113702. doi: 10.1063/1.4828656.
This paper describes the development of a versatile coherent x-ray diffraction microscope capable of imaging biological specimens in solution. The microscope is a flexible platform accommodating various conditions, from low vacuum (10(-2) Pa) to helium gas filled ambient pressure. This flexibility greatly expands the application area, from in situ materials science to biology systems in their native state, by significantly relaxing restrictions to the sample environment. The coherent diffraction microscope has been used successfully to image a yeast cell immersed in buffer solution. We believe that the design of this coherent diffraction microscope can be directly adapted to various platforms such as table top soft x-ray laser, synchrotron x-ray sources, and x-ray free electron laser with minor relevant adjustments.
本文描述了一种多功能相干X射线衍射显微镜的研制，该显微镜能够对溶液中的生物样本进行成像。该显微镜是一个灵活的平台，可适应各种条件，从低真空（10^(-2) 帕）到充满氦气的环境压力。这种灵活性极大地扩展了应用领域，从原位材料科学到天然状态的生物系统，通过显著放宽对样品环境的限制。相干衍射显微镜已成功用于对浸入缓冲溶液中的酵母细胞进行成像。我们相信，这种相干衍射显微镜的设计经过少量相关调整后可直接应用于各种平台，如桌面软X射线激光器、同步辐射X射线源和X射线自由电子激光器。
