Liu Huimin, He Xiuyuan, Song Xiaofeng, Xu Liang, Zhang Yun, Zhou Guoli, Zhu Wenjiao, Chang Chen, Yin Zhian, Shi Yuhang, Wang Chuanqing, Chang Hongtao
College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China.
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, China.
Infect Genet Evol. 2016 Jun;40:67-72. doi: 10.1016/j.meegid.2016.02.025. Epub 2016 Feb 23.
Encephalomyocarditis virus (EMCV) can infect many host species and cause acute myocarditis and respiratory failure in piglets, reproductive failure in pregnant sows. In this study, an EMCV strain, designated JZ1202, was isolated from semi-captive wild boars that presented with acute myocarditis and sudden death in central China. It was identified by hemagglutination inhibition (HI) assay, reverse transcription polymerase chain reaction (RT-PCR) and genome sequencing. The subsequent results showed that the virus could produce a specific cytopathic effect on BHK cells and could cause clinical symptoms and pathological changes in mice. Complete genome sequencing and multiple sequence alignment indicated that JZ1202 strain was 81.3%-99.9% identical with other isolates worldwide. Phylogenetic analysis of the whole genome, ORF, VP3/VP1 and 3D genes using neighbor-joining method revealed that JZ1202 isolate was grouped into lineage 1. The results of this study confirmed that an EMCV strain JZ 1202 isolated from wild boar in central China was fatal to mice and provided new epidemiologic data on EMCV in China.
脑心肌炎病毒（EMCV）可感染多种宿主物种，导致仔猪急性心肌炎和呼吸衰竭，妊娠母猪繁殖失败。在本研究中，从中国中部出现急性心肌炎和猝死的半圈养野猪中分离出一株EMCV毒株，命名为JZ1202。通过血凝抑制（HI）试验、逆转录聚合酶链反应（RT-PCR）和基因组测序对其进行鉴定。随后的结果表明，该病毒可对BHK细胞产生特异性细胞病变效应，并可在小鼠中引起临床症状和病理变化。全基因组测序和多序列比对表明，JZ1202毒株与全球其他分离株的同源性为81.3%-99.9%。使用邻接法对全基因组、开放阅读框、VP3/VP1和3D基因进行系统发育分析，结果显示JZ1202分离株属于第1分支。本研究结果证实，从中国中部野猪中分离出的EMCV毒株JZ 1202对小鼠具有致死性，并提供了中国EMCV的新流行病学数据。
