Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699, USA.
Analyst. 2014 Feb 7;139(3):559-63. doi: 10.1039/c3an02055j.
A new biocatalytic assay analyzing the simultaneous presence of creatine kinase (CK) and alanine transaminase (ALT) was developed aiming at the recognition of biofluids of different gender for forensic applications. Knowing the difference in the concentrations of CK and ALT enzymes in the blood of healthy adults of male and female groups we mimicked the samples of different gender with various CK-ALT concentrations. The analysis was performed using a multi-enzyme/multi-step biocatalytic cascade where the differences in both included enzymes resulted in an amplified difference in the final analytical response. The analysis performed in human serum solutions allowed discrimination of samples corresponding to male/female groups. The robustness of the developed analysis allowed determination of the gender for serum solutions after their drying and ageing at least for 1 hour. Importantly for forensic applications, reaction with a chromogenic reactant nitroblue tetrazolium allowed qualitative discrimination of the "male" and "female" samples by the naked eye.
一种新的生物催化分析方法分析肌酸激酶（CK）和丙氨酸氨基转移酶（ALT）的同时存在，旨在识别法医应用中不同性别的生物液体。由于知道健康成年男性和女性群体血液中 CK 和 ALT 酶浓度的差异，我们用不同 CK-ALT 浓度模拟了不同性别的样本。该分析采用多酶/多步生物催化级联反应，其中两种包含的酶的差异导致最终分析响应的放大差异。在人血清溶液中的分析允许区分对应于男性/女性组的样本。所开发的分析的稳健性允许在干燥和老化至少 1 小时后确定血清溶液的性别。重要的是，对于法医应用，与显色反应物硝基蓝四唑的反应允许通过肉眼定性区分“男性”和“女性”样本。
