College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China; Bioenergy Research Institute, Nanjing University of Technology, Nanjing 210095, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China.
Bioresour Technol. 2014;152:220-4. doi: 10.1016/j.biortech.2013.10.086. Epub 2013 Nov 5.
Microbial fuel cells (MFCs) are promising for generating bioenergy and treating organic waste simultaneously. However, low extracellular electron transfer (EET) efficiency between electrogens and anodes remains one of the major bottlenecks in practical applications of MFCs. In this paper, pyocyanin (PYO) synthesis pathway was manipulated to improve the EET efficiency in Pseudomonas aeruginosa-inoculated MFCs. By overexpression of phzM (methyltransferase encoding gene), the maximum power density of P. aeruginosa-phzM-inoculated MFC was enhanced to 166.68 μW/cm(2), which was four folds of the original strain. In addition, the phzM overexpression strain exhibited an increase of 1.6 folds in PYO production and about a onefold decrease in the total internal resistance than the original strain, which should underlie the enhancement of the EET efficiency and the electricity power output (EPT). On the basis of these results, the manipulation of electron shuttles synthesis pathways could be an efficient approach to improve the EPT of MFCs.
微生物燃料电池（MFCs）在同时产生生物能源和处理有机废物方面具有广阔的前景。然而，在微生物燃料电池的实际应用中，电子供体和阳极之间的细胞外电子传递（EET）效率仍然是主要瓶颈之一。在本文中，通过操纵铜绿假单胞菌（Pseudomonas aeruginosa）中吡咯喹啉醌（PYO）的合成途径来提高电子传递效率。通过过表达 phzM（编码甲基转移酶的基因），接种有 P. aeruginosa-phzM 的 MFC 的最大功率密度提高到 166.68 μW/cm(2)，是原始菌株的四倍。此外，与原始菌株相比，phzM 过表达菌株的 PYO 产量增加了 1.6 倍，总内阻降低了约 1 倍，这应该是 EET 效率和电力输出（EPT）提高的基础。基于这些结果，操纵电子穿梭合成途径可能是提高 MFCs 的 EPT 的有效方法。
