College of Biotechnology and Pharmaceutical Engineering, Bioenergy Research Institute, Nanjing TECH University, Nanjing 211816, China.
Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China.
Bioresour Technol. 2017 Oct;241:1191-1196. doi: 10.1016/j.biortech.2017.06.050. Epub 2017 Jun 12.
Microbial fuel cell (MFC) is a promising device for energy generation and organic waste treatment simultaneously by electrochemically active bacteria (EAB). In this study, an integrated aerobic-anaerobic strategy was developed to improve the performance of P. aeruginosa-inoculated MFC. With an aerobic start-up and following an anaerobic discharge process, the current density of MFC reached a maximum of 99.80µA/cm, which was 91.6% higher than the MFC with conventional constant-anaerobic operation. Cyclic voltammetry and HPLC analysis showed that aerobic start-up significantly increased electron shuttle (pyocyanin) production (76% higher than the constant-anaerobic MFC). Additionally, enhanced anode biofilm formation was also observed in the integrated aerobic-anaerobic MFC. The increased pyocyanin production and biofilm formation promoted extracellular electron transfer from EAB to the anode and were the underlying mechanism for the MFC performance enhancement. This work demonstrated the integrated aerobic-anaerobic strategy would be a practical strategy to enhance the electricity generation of MFC.
微生物燃料电池（MFC）是一种很有前途的设备，可通过电化学活性细菌（EAB）同时进行能源发电和有机废物处理。在这项研究中，开发了一种集成的需氧-厌氧策略，以提高接种铜绿假单胞菌的 MFC 的性能。通过需氧启动和随后的厌氧排放过程，MFC 的电流密度达到了 99.80µA/cm 的最大值，比传统的恒厌氧操作的 MFC 高 91.6%。循环伏安法和高效液相色谱分析表明，需氧启动显著增加了电子穿梭体（绿脓菌素）的产生（比恒厌氧 MFC 高 76%）。此外，在集成的需氧-厌氧 MFC 中也观察到了增强的阳极生物膜形成。增加的绿脓菌素产生和生物膜形成促进了 EAB 到阳极的细胞外电子转移，这是 MFC 性能增强的潜在机制。这项工作表明，集成的需氧-厌氧策略将是增强 MFC 发电的一种实用策略。
