Wu Wenguo, Hong Huiya, Lin Jia, Yang Dayun
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.
Fujian Key Laboratory of Translational Research in Cancer and Neurodegenerative Diseases, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350108, China.
Biosensors (Basel). 2024 Dec 11;14(12):606. doi: 10.3390/bios14120606.
Simultaneous monitoring of antimicrobial responses to bacterial metabolic activity and biofilm formation is critical for efficient screening of new anti-biofilm drugs. A microbial fuel cell-based biosensor using as an electricigen was constructed. The effects of silver nanoparticles (AgNPs) on the cellular metabolic activity and biofilm formation of in the biosensors were investigated and compared with the traditional biofilm detection method. The crystal violet staining results showed that the concentration of AgNPs being increased to 20 and 40 μg/mL had a slight and obvious inhibitory effect on biofilm formation, respectively. In comparison, the detection sensitivity of the biosensor was much higher. When the concentration of AgNPs was 5 μg/mL, the output voltage of the biosensor was suppressed, and the inhibition gradually increased with the AgNPs dose. AgNPs inhibited the activity of planktonic cells in the anolyte and the formation of biofilm on the anode surface, and it had a dose-dependent effect on the secretion of phenazine in the anolyte. The biosensor could monitor the impacts of AgNPs not only on biofilm formation but also on cell activity and metabolic activity. It provides a new and sensitive method for the screening of anti-biofilm drugs.
同时监测抗菌药物对细菌代谢活性和生物膜形成的反应对于高效筛选新型抗生物膜药物至关重要。构建了一种以 作为产电微生物的基于微生物燃料电池的生物传感器。研究了银纳米颗粒（AgNPs）对生物传感器中 的细胞代谢活性和生物膜形成的影响，并与传统生物膜检测方法进行了比较。结晶紫染色结果表明，AgNPs浓度增加到20 μg/mL和40 μg/mL时，分别对生物膜形成有轻微和明显的抑制作用。相比之下，生物传感器的检测灵敏度要高得多。当AgNPs浓度为5 μg/mL时，生物传感器的输出电压受到抑制，且抑制作用随AgNPs剂量增加而逐渐增强。AgNPs抑制了阳极液中浮游细胞的活性以及阳极表面生物膜的形成，并且对阳极液中吩嗪的分泌具有剂量依赖性影响。该生物传感器不仅可以监测AgNPs对生物膜形成的影响，还可以监测其对细胞活性和代谢活性的影响。它为抗生物膜药物的筛选提供了一种新的、灵敏的方法。
