Veterinary Medical Center, Chungbuk National University, 361-763, Republic of Korea.
In Vivo. 2013 Nov-Dec;27(6):755-60.
Although acellular dermal matrix (ADM) is a widely used graft material for abdominal wall repair, differences in processing methods might yield different healing activities. The aim of this study was to compare the healing effects of two human-derived ADM prototypes in abdominal wall reconstruction.
A standardized 15×50 mm abdominal wall defect was created in 28 Sprague-Dawley rats, which were then implanted with either an EDTA-treated ADM prototype or a salt/solvent-treated ADM prototype. Adhesion formation, tensile strength, tissue ingrowth, neovascularization and inflammatory cell infiltration were then assessed in the two ADM prototypes during the experimental period.
In both ADM prototypes, mild adhesion formation with the omentum was observed at autopsy at one and four weeks post-implantation. Tensile strength was higher at four weeks post-implantation than that at one week post-implantation. Good neovascularization was observed in the periphery of the ADM, but not in the ADM core. Muscles facing the ADM and muscle-ADM junctions were thick and long at one week post-implantation and had been replaced by new host collagen at four weeks post-implantation. No mesothelial cells at the margins were observed at one and four weeks post-implantation. The thickness of the remaining implanted ADM at four weeks post-implantation was less than that at one week post-implantation. There were no statistical differences between the two ADM prototypes in terms of adhesion formation, tensile strength, tissue ingrowth, neovascularization and inflammatory cell infiltration during the experimental period.
These results indicate that both ADM prototypes are applicable implant materials for repair of abdominal wall defects.
尽管脱细胞真皮基质（ADM）是一种广泛用于腹壁修复的移植物材料，但不同的处理方法可能会产生不同的愈合活性。本研究旨在比较两种人源 ADM 原型在腹壁重建中的愈合效果。
在 28 只 Sprague-Dawley 大鼠中创建了一个标准化的 15×50mm 腹壁缺损，然后将其植入 EDTA 处理的 ADM 原型或盐/溶剂处理的 ADM 原型中。然后在实验期间评估两种 ADM 原型中的粘连形成、拉伸强度、组织内生长、新生血管形成和炎症细胞浸润。
在两种 ADM 原型中，在植入后 1 周和 4 周的尸检中，均观察到与大网膜轻度粘连。植入后 4 周的拉伸强度高于植入后 1 周。在 ADM 的外围观察到良好的新生血管形成，但在 ADM 核心中没有。植入后 1 周，面对 ADM 的肌肉和肌肉-ADM 交界处较厚且较长，植入后 4 周已被新的宿主胶原取代。在植入后 1 周和 4 周时，均未观察到 ADM 边缘的间皮细胞。植入后 4 周时，剩余植入 ADM 的厚度小于植入后 1 周时。在实验期间，两种 ADM 原型在粘连形成、拉伸强度、组织内生长、新生血管形成和炎症细胞浸润方面均无统计学差异。
这些结果表明，两种 ADM 原型均适用于修复腹壁缺损的植入材料。
