Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, Republic of Korea.
L&C BIO Co./R&D Center, SK Techno Park Bizcenter 2F 201, 190-2, Sangdaewon-dong, Joongwon-gu, Seongnam-Si, Gyeonggi-do, Republic of Korea.
Biomaterials. 2015 Mar;44:195-205. doi: 10.1016/j.biomaterials.2014.12.004. Epub 2015 Jan 14.
Here, we describe a novel human acellular dermal matrix (ADM) cross-linked using electron beam irradiation. Structural and biomechanical characteristics of the human ADM were assessed by infrared spectrometry and uni-axial tensile testing. Electron beam irradiation affects collagen secondary structure, which can be detected in the amide I spectral region (1660 cm(-1) and 1690 cm(-1)). At doses exceeding 25 kGy, cross-linking of the collagen matrix results in a denser, more stratified appearance and parallel arrangement, with significantly increased tensile strength and elastic modulus. In a micropig model, the implanted ADM elicits rapid host cell infiltration and extracellular matrix deposition; however, the delayed remodeling resulted in long-term structural integrity. Furthermore, mean densities of collagen and elastin, expression of extracellular matrix proteins, and microvessel formation within the implanted ADM increased significantly, whereas the thickness of the implanted ADM did not decrease during the course of the study. Compared with normal adjacent tissue, type I collagen mRNA levels in the ADM increased 12-fold at 3 months after implantation, and transforming growth factor-β mRNA levels increased 3.3-fold at 2 months. Matrix metalloproteinase (MMP)-1 and MMP-9 mRNA levels were also elevated. Collectively, these results demonstrate that the structural and biomechanical properties of this novel cross-linked human ADM are adequate for use as a biologic tissue substitute.
在这里，我们描述了一种新型的人去细胞真皮基质（ADM），它是通过电子束辐照交联的。通过红外光谱和单轴拉伸试验评估了人 ADM 的结构和生物力学特性。电子束辐照会影响胶原的二级结构，这可以在酰胺 I 光谱区域（1660cm(-1) 和 1690cm(-1)）中检测到。在剂量超过 25kGy 时，胶原基质的交联会导致更致密、更分层的外观和平行排列，同时显著提高拉伸强度和弹性模量。在小型猪模型中，植入的 ADM 会迅速引起宿主细胞浸润和细胞外基质沉积；然而，延迟的重塑导致了长期的结构完整性。此外，植入 ADM 内的胶原和弹性蛋白的平均密度、细胞外基质蛋白的表达以及微血管形成显著增加，而植入 ADM 的厚度在研究过程中并没有减少。与正常相邻组织相比，植入后 3 个月 ADM 中的 I 型胶原 mRNA 水平增加了 12 倍，转化生长因子-β mRNA 水平增加了 3.3 倍。基质金属蛋白酶（MMP）-1 和 MMP-9 的 mRNA 水平也升高。总的来说，这些结果表明，这种新型交联人 ADM 的结构和生物力学特性足以用作生物组织替代品。
