Laboratório de Doenças Infecciosas, Setor Micologia, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Braz J Microbiol. 2013 Oct 30;44(2):481-4. doi: 10.1590/S1517-83822013000200024. eCollection 2013.
Fungi of the Aspergillus genus are widespread and contaminate the environment. Thousands of conidia are released from each phialide and dispersed in the air every day. These fungi are considered important mycose-causing agents in hospitals. Due to this, research to determine prevalent fungi from the Aspergillus genus in hospital environments, and an adequate disinfection program in these areas is are needed. This study evaluated the susceptibility of Aspergillus spp. isolated from a veterinary environment against four disinfectants. Successive dilutions of disinfectants (log2) were used according to CLSI M38-A2 microdilution technique adapted to chemical agents against 18 isolates of this genus. After 72 hours of incubation, the Minimum Inhibiting Concentration and Minimum Fungicidal Concentration capable of inhibiting 50% and 90% of the isolates were determined. Chlorexidine-cetrimine, benzalconium chloride and a chlorophenol derivative proved to be effective against all isolates with a lower MIC than that suggested by the manufacturer, except for the A. flavus strain. Sodium hypochlorite was ineffective against three A. fumigatus, three A. flavus and one A. niger isolate. These results demonstrated that all studied disinfectants were effective against environmental isolates, with the exception of sodium hypochlorite, which showed lower effectiveness.
曲霉属真菌分布广泛，会污染环境。每个瓶梗每天都会释放数千个分生孢子，并在空气中传播。这些真菌被认为是医院中重要的致病真菌。因此，需要研究确定医院环境中曲霉属真菌的流行情况，并制定适当的消毒方案。本研究评估了从兽医环境中分离出的曲霉属真菌对四种消毒剂的敏感性。根据 CLSI M38-A2 微稀释技术，针对 18 株该属真菌，采用连续稀释消毒剂（log2）的方法。孵育 72 小时后，确定了能够抑制 50%和 90%分离株的最低抑菌浓度和最低杀菌浓度。氯己定-十六烷基二甲基溴化铵和一种氯苯酚衍生物对所有分离株均有效，MIC 均低于制造商建议值，但黄曲霉株除外。次氯酸钠对 3 株烟曲霉、3 株黄曲霉和 1 株黑曲霉分离株无效。这些结果表明，所有研究的消毒剂对环境分离株均有效，除次氯酸钠外，次氯酸钠的效果较低。
