Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Ilha do Fundão, Rio de Janeiro, RJ, Brazil. ; Department of Microbial Ecology, University of Groningen, Kerklaan, Haren, The Netherlands.
Braz J Microbiol. 2013 Oct 30;44(2):633-41. doi: 10.1590/S1517-83822013000200044. eCollection 2013.
The petroleum-derived degrading Dietzia cinnamea strain P4 recently had its genome sequenced and annotated. This allowed employing the data on genes that are involved in the degradation of n-alkanes. To examine the physiological behavior of strain P4 in the presence of n-alkanes, the strain was grown under varying conditions of pH and temperature. D. cinnamea P4 was able to grow at pH 7.0-9.0 and at temperatures ranging from 35 ºC to 45 ºC. Experiments of gene expression by real-time quantitative RT-PCR throughout the complete growth cycle clearly indicated the induction of the regulatory gene alkU (TetR family) during early growth. During the logarithmic phase, a large increase in transcriptional levels of a lipid transporter gene was noted. Also, the expression of a gene that encodes the protein fused rubredoxin-alkane monooxygenase was enhanced. Both genes are probably under the influence of the AlkU regulator.
最近对源自石油的降解狄氏枝霉 P4 菌株进行了基因组测序和注释。这使得可以利用与降解正烷烃相关的基因数据。为了研究 P4 菌株在存在正烷烃时的生理行为，在不同 pH 值和温度条件下培养该菌株。狄氏枝霉 P4 能够在 pH 值为 7.0-9.0 和温度范围为 35 ºC 至 45 ºC 的条件下生长。通过实时定量 RT-PCR 进行的整个生长周期的基因表达实验清楚地表明，在早期生长过程中，调节基因 alkU（TetR 家族）被诱导。在对数生长期，注意到脂质转运基因的转录水平大幅增加。此外，编码融合 rubredoxin-烷烃单加氧酶的蛋白质的基因的表达也增强了。这两个基因可能都受到 AlkU 调节剂的影响。
