坏死性小肠结肠炎与轮状病毒感染的偶合及与细胞因子的潜在关联。
The coincidence of necrotizing enterocolitis and rotavirus infections and potential associations with cytokines.
机构信息
Department of Pediatrics, Çukurova University, Adana, Turkey;
出版信息
Can J Infect Dis Med Microbiol. 2012 Winter;23(4):e103-5. doi: 10.1155/2012/530309.
BACKGROUND
Necrotizing enterocolitis (NEC) is the most common gastrointestinal disease in neonatal intensive care units. Although the pathogenesis of NEC remains unclear, evidence suggests that infections, especially bacterial infections, may play an important role. Viral infections may also result in NEC. Several outbreaks of NEC associated with rotaviruses have been described previously.
OBJECTIVE
To investigate the association between rotavirus (RV) and serum interleukin (IL)-6 and IL-8 levels in infants with NEC.
METHODS
RV infections were prospectively studied using antigen detection in the stools of 31 infants with NEC. Additionally, serum levels of IL-6, IL-8 and tumour necrosis factor-alpha were tested using micro-ELISA at 0 h and 48 h after diagnosis of NEC.
RESULTS
Fecal specimens from 13 infants were positive, while fecal specimens from 18 infants were negative for RV according to antigen detection (RV+ and RV- groups, respectively). The mortality rate and the severity of NEC were not significantly different between the RV+ and RV- groups. IL-6 levels at 0 h and 48 h after diagnosis of NEC in RV+ infants were lower compared with RV- infants, while IL-8 levels were greater at 0 h and 48 h after diagnosis of NEC in RV+ infants compared with RV- infants.
CONCLUSION
A high prevalence of RV infection in neonates with NEC was found. Decreased IL-6 levels and increased IL-8 and tumour necrosis factor-alpha levels in RV+ neonates with NEC suggests a role for RV in NEC.
背景
坏死性小肠结肠炎（NEC）是新生儿重症监护病房中最常见的胃肠道疾病。尽管 NEC 的发病机制仍不清楚，但有证据表明感染，尤其是细菌感染，可能发挥重要作用。病毒感染也可能导致 NEC。先前已经描述了几起与轮状病毒相关的 NEC 爆发。
目的
研究轮状病毒（RV）与 NEC 婴儿血清白细胞介素（IL）-6 和 IL-8 水平之间的关系。
方法
采用粪便抗原检测法前瞻性研究 31 例 NEC 婴儿的 RV 感染。此外，在 NEC 诊断后 0 h 和 48 h 时，使用微 ELISA 检测血清中 IL-6、IL-8 和肿瘤坏死因子-α的水平。
结果
根据抗原检测，13 例婴儿粪便标本为阳性，18 例婴儿粪便标本为阴性（RV+组和 RV-组）。RV+组和 RV-组的死亡率和 NEC 严重程度无显著差异。RV+婴儿 NEC 诊断后 0 h 和 48 h 的 IL-6 水平低于 RV-婴儿，而 IL-8 水平在 RV+婴儿 NEC 诊断后 0 h 和 48 h 高于 RV-婴儿。
结论
在 NEC 新生儿中发现 RV 感染的高患病率。NEC 中 RV+婴儿的 IL-6 水平降低，IL-8 和肿瘤坏死因子-α水平升高提示 RV 在 NEC 中发挥作用。
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