Kim Kyung-Hyun, Lee Woo-Ram, An Hyun-Jin, Kim Jung-Yeon, Chung Hyun, Han Sang-Mi, Lee Myeong-Lyoel, Lee Kwang-Gill, Pak Sok Cheon, Park Kwan-Kyu
Department of Pathology, Catholic University of Daegu, College of Medicine Daegu, 705-718, Republic of Korea.
Int J Clin Exp Pathol. 2013 Nov 15;6(12):2896-903. eCollection 2013.
Honeybee (Apis mellifera L.) venom (BV) has been traditionally used for the treatment of pain and inflammatory diseases such as itchy skin problems. However, the precise mechanism of BV in ameliorating the scratching behavior is not fully understood.
In order to evaluate the effect of BV on atopic dermatitis-related symptoms in mice, we used a mouse skin scratching model induced by compound 48/80. The anti-itch effect of BV was investigated in a compound 48/80-induced mouse scratching behavior model. BALB/c mice were injected intraperitoneally with vehicle (saline 0.9%) or BV (0.01 and 0.1 mg/kg). One hour after treatment, the animals received a subcutaneous injection of compound 48/80. Intraperitoneal administration of BV (0.01 and 0.1 mg/kg) attenuated compound 48/80-induced scratching behaviors. The anti-scratching behavior effect of BV was in proportional to its vascular permeability effects. Treatment with BV also inhibited the degranulation of mast cells and the production of pro-inflammatory cytokines in compound 48/80-treated skin tissues. According to these results, BV may improve atopic dermatitis-related symptoms by inhibiting the mast cell degranulation and pro-inflammatory cytokine expression.
传统上，蜜蜂（意大利蜜蜂）毒液（BV）用于治疗疼痛和炎症性疾病，如皮肤瘙痒问题。然而，BV改善抓挠行为的确切机制尚不完全清楚。
为了评估BV对小鼠特应性皮炎相关症状的影响，我们使用了由化合物48/80诱导的小鼠皮肤抓挠模型。在化合物48/80诱导的小鼠抓挠行为模型中研究了BV的止痒效果。将BALB/c小鼠腹腔注射赋形剂（0.9%生理盐水）或BV（0.01和0.1mg/kg）。治疗1小时后，动物皮下注射化合物48/80。腹腔注射BV（0.01和0.1mg/kg）可减轻化合物48/80诱导的抓挠行为。BV的抗抓挠行为作用与其血管通透性作用成正比。BV治疗还抑制了化合物48/80处理的皮肤组织中肥大细胞的脱颗粒和促炎细胞因子的产生。根据这些结果，BV可能通过抑制肥大细胞脱颗粒和促炎细胞因子表达来改善特应性皮炎相关症状。
