Coulson B S, Tursi J M, McAdam W J, Bishop R F
Virology. 1986 Oct 30;154(2):302-12. doi: 10.1016/0042-6822(86)90456-3.
Neutralizing monoclonal antibodies were derived to human rotaviruses RV-4 (serotype 1), RV-5 (serotype 2), and ST-3 (serotype 4). By enzyme immunoassay and fluorescent focus neutralization, eight of the antibodies appeared to be specific for the immunizing serotype, and so have potential as reagents for rotavirus serotyping by enzyme immunoassay. Seven of these were shown by Western blotting, enzyme immunoassay for antibody additivity, and reaction with rotavirus reassortants, to be directed against the major outer capsid glycoprotein. The remaining serotype-specific antibody immunoprecipitated the 84-kD outer capsid protein. One antibody reacted with all serotype 1 and 3 rotaviruses but not with serotypes 2 or 4. When tested with virus mutants, this antibody recognized an immunodominant determinant of neutralization shared between serotypes 1 and 3 on the major outer shell glycoprotein. Our results suggest that two outer capsid proteins possess determinants of neutralization, and that viruses of different serotypes may share immunodominant neutralization sites.
针对人轮状病毒RV - 4（血清型1）、RV - 5（血清型2）和ST - 3（血清型4）制备了中和性单克隆抗体。通过酶免疫测定和荧光灶中和试验，其中8种抗体似乎对免疫血清型具有特异性，因此有潜力作为酶免疫测定法进行轮状病毒血清分型的试剂。通过蛋白质印迹法、抗体加性酶免疫测定以及与轮状病毒重配株的反应，证实其中7种抗体针对主要外衣壳糖蛋白。其余血清型特异性抗体免疫沉淀了84-kD外衣壳蛋白。一种抗体与所有血清型1和3的轮状病毒反应，但不与血清型2或4的轮状病毒反应。在用病毒突变体进行测试时，该抗体识别主要外壳糖蛋白上血清型1和3之间共有的中和免疫显性决定簇。我们的结果表明，两种外衣壳蛋白具有中和决定簇，不同血清型的病毒可能共享免疫显性中和位点。
