Taniguchi K, Urasawa T, Morita Y, Greenberg H B, Urasawa S
J Infect Dis. 1987 Jun;155(6):1159-66. doi: 10.1093/infdis/155.6.1159.
Four serotypes of human rotaviruses that can be differentiated by neutralization tests have been described so far. We prepared serotype 1-, 2-, 3-, and 4-specific, neutralizing monoclonal antibodies to human rotaviruses. All were directed to VP7, a glycoprotein that carries a major serotype specificity. An enzyme-linked immunosorbent assay using these monoclonal antibodies has been developed for serotyping human rotavirus isolates. The sensitivity and specificity of this method were verified by using various strains of human rotavirus that were adapted to cell culture. Furthermore, it was shown that the method was applicable to serotyping human rotaviruses directly in stool specimens.
到目前为止，已经描述了四种可通过中和试验区分的人类轮状病毒血清型。我们制备了针对人类轮状病毒的1型、2型、3型和4型特异性中和单克隆抗体。所有抗体均针对VP7，VP7是一种具有主要血清型特异性的糖蛋白。利用这些单克隆抗体开发了一种酶联免疫吸附测定法，用于对人类轮状病毒分离株进行血清分型。通过使用适应细胞培养的各种人类轮状病毒株，验证了该方法的敏感性和特异性。此外，还表明该方法可直接用于粪便标本中人类轮状病毒的血清分型。
