Nagesha H S, Brown L E, Holmes I H
School of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
J Virol. 1989 Aug;63(8):3545-9. doi: 10.1128/JVI.63.8.3545-3549.1989.
Using three serotypes (four strains) of cultivable porcine rotavirus as immunizing antigens, 10 neutralizing monoclonal antibodies were characterized. One VP4-specific monoclonal antibody directed against porcine rotavirus BEN-144 (serotype G4) neutralized human rotavirus strain ST-3 in addition to the homologous porcine virus. All nine VP7-specific monoclonal antibodies were highly specific for viruses of the same serotype as the immunizing rotavirus strain. One exception was the VP7-specific monoclonal antibody C3/1, which neutralized both serotype G3 and G5 rotaviruses. However, this monoclonal antibody did not neutralize the porcine rotavirus AT/76, also of serotype G3, nor mutants of SA-11 virus (serotype G3) which were selected with monoclonal antibody A10/N3 and are known to have mutations affecting the C antigenic region.
以三种可培养的猪轮状病毒血清型（四个毒株）作为免疫抗原，对10株中和性单克隆抗体进行了特性鉴定。一种针对猪轮状病毒BEN - 144（G4血清型）的VP4特异性单克隆抗体，除了能中和同源猪病毒外，还能中和人轮状病毒ST - 3株。所有九株VP7特异性单克隆抗体对与免疫轮状病毒株相同血清型的病毒具有高度特异性。一个例外是VP7特异性单克隆抗体C3/1，它能中和G3和G5血清型轮状病毒。然而，该单克隆抗体不能中和同样为G3血清型的猪轮状病毒AT/76，也不能中和用单克隆抗体A10/N3筛选出的、已知具有影响C抗原区域突变的SA - 11病毒（G3血清型）突变体。
