Yamamoto M
Angiology. 1986 Sep;37(9):663-71. doi: 10.1177/000331978603700908.
The effects of fibrinogen, globulin, albumin, and packed blood cell volume (hematocrit) on the kinetics of erythrocyte aggregation (RBC-A) after stasis were quantitatively evaluated by recording the changes of light transmission through the blood in transparent tubing with an apparatus consisting of a light-emitting diode and a silicon photodiode. The measurements were done on samples of heparinized whole blood, suspensions of erythrocytes containing various concentrations of fibrinogen (0.5 to 25 g/L) or gamma globulin (25 to 150 g/L), and blood of various hematocrit values prepared from three samples of whole blood having different concentrations of fibrinogen. The half-time of the increase of light transmission after the flow-stop was used as an index of the kinetics of RBC-A (T1/2). This simple and rapid method for estimating erythrocyte aggregation had excellent reproducibility (N = 51, r = 0.99). The values of T1/2 showed significant negative correlations with the concentrations of fibrinogen and globulin but a positive correlation with that of albumin. The T1/2 value decreased markedly and then remained almost constant with increase in the value of hematocrit, but the influence of hematocrit on T1/2 was markedly dependent on the concentration of fibrinogen.
通过使用由发光二极管和硅光电二极管组成的仪器记录透明管中血液透光率的变化，对纤维蛋白原、球蛋白、白蛋白和红细胞压积对血液淤滞后红细胞聚集动力学（RBC-A）的影响进行了定量评估。测量是在肝素化全血样本、含有不同浓度纤维蛋白原（0.5至25 g/L）或γ球蛋白（25至150 g/L）的红细胞悬液以及由三个不同纤维蛋白原浓度的全血样本制备的不同红细胞压积值的血液上进行的。血流停止后透光率增加的半衰期用作RBC-A动力学指标（T1/2）。这种估算红细胞聚集的简单快速方法具有出色的重现性（N = 51，r = 0.99）。T1/2值与纤维蛋白原和球蛋白浓度呈显著负相关，但与白蛋白浓度呈正相关。随着红细胞压积值增加，T1/2值显著下降，然后几乎保持恒定，但红细胞压积对T1/2的影响明显取决于纤维蛋白原浓度。
