采用超高效液相色谱-串联质谱法检测产碳青霉烯酶细菌。
Detection of carbapenemase-producing bacteria by using an ultra-performance liquid chromatography-tandem mass spectrometry method.
机构信息
GIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), University of Lyon, Saint-Etienne, France.
出版信息
Antimicrob Agents Chemother. 2014;58(2):1231-4. doi: 10.1128/AAC.01540-13. Epub 2013 Dec 2.
Abstract
The emergence of carbapenemase-producing bacteria poses a new challenge in the management of antibiotic therapies for patients. This report describes a new method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for rapid detection of carbapenemase activity in enterobacteria, Pseudomonas aeruginosa, and Acinetobacter baumannii. In a panel of 78 isolates, including 41 carbapenemase-producing strains, the ULPC-MS/MS assay showed 100% agreement with molecular characterization, whereas six carbapenemase-producing isolates were not detected by the modified Hodge test.
摘要
产碳青霉烯酶细菌的出现给患者的抗生素治疗管理带来了新的挑战。本报告描述了一种使用超高效液相色谱-串联质谱（UPLC-MS/MS）快速检测肠杆菌科、铜绿假单胞菌和鲍曼不动杆菌中产碳青霉烯酶活性的新方法。在包括 41 株产碳青霉烯酶菌株在内的 78 株分离株的检测中，该 UPLC-MS/MS 检测法与分子特征分析完全一致，而改良 Hodge 试验未能检测到 6 株产碳青霉烯酶的分离株。
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