抗蛋白酶剂可抑制甲病毒在培养细胞和受感染动物中的复制。
Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents.
作者信息
Zhirnov O P, Ovcharenko A V, Melnikova E E, Bukrinskaya A G
出版信息
Antiviral Res. 1986 Aug;6(5):255-65. doi: 10.1016/0166-3542(86)90021-5.
Abstract
The influence of different antiproteinase agents on alphavirus replication was examined. Sindbis virus multicycle replication in cultured cells was suppressed by N-tosyl-phenylalanine chloromethyl ketone (TPCK), an inhibitor of chymotrypsin-like proteinases, and by aprotinin, an inhibitor of a wide spectrum of proteinases. Antiviral activity of TPCK was also demonstrated in Sindbis virus-infected animals. Parenteral injections of TPCK in infected mice reduced virus titers in brain and blood. The possible mechanism(s) of antiviral action of the antiproteinase agents are discussed.
摘要
研究了不同抗蛋白酶剂对甲病毒复制的影响。在培养细胞中，糜蛋白酶样蛋白酶抑制剂N-对甲苯磺酰苯丙氨酸氯甲基酮（TPCK）和广谱蛋白酶抑制剂抑肽酶可抑制辛德毕斯病毒的多轮复制。TPCK在感染辛德毕斯病毒的动物中也表现出抗病毒活性。给感染的小鼠腹腔注射TPCK可降低脑和血液中的病毒滴度。文中讨论了抗蛋白酶剂抗病毒作用的可能机制。
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Direct evidence for the presence of histidine in the active center of chymotrypsin.关于组氨酸存在于胰凝乳蛋白酶活性中心的直接证据。
Biochemistry. 1963 Mar-Apr;2:252-5. doi: 10.1021/bi00902a008.
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Diabetes responsive to intravenous but not subcutaneous insulin: effectiveness of aprotinin.对静脉注射胰岛素有反应但对皮下注射胰岛素无反应的糖尿病：抑肽酶的有效性
N Engl J Med. 1981 Aug 13;305(7):363-8. doi: 10.1056/NEJM198108133050702.
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Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins.辛德毕斯病毒26S mRNA的核苷酸序列及编码的病毒结构蛋白的推导序列。
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Synthesis and processing of Sindbis virus nonstructural proteins in vitro.辛德毕斯病毒非结构蛋白的体外合成与加工
Virology. 1982 Apr 30;118(2):363-79. doi: 10.1016/0042-6822(82)90356-7.
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J Virol. 1983 Jul;47(1):46-54. doi: 10.1128/JVI.47.1.46-54.1983.
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