浒苔多糖增强正常小鼠的免疫力。
Polysaccharides from Enteromorpha prolifera enhance the immunity of normal mice.
机构信息
Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; Center of Resource Chemical & New Material, Qingdao, Qingdao 266100, PR China.
Mariculture Institute of Shandong Province, Qingdao 266002, PR China.
出版信息
Int J Biol Macromol. 2014 Mar;64:1-5. doi: 10.1016/j.ijbiomac.2013.11.013. Epub 2013 Dec 1.
The effects of polysaccharides from Enteromorpha prolifera (PEP) on cell-mediated immunity, humoral immunity and mononuclear phagocytic system function were evaluated to assess the immunomodulatory potential of these macromolecules. Relevant immunological mechanisms were verified by biochemical assays and western blot analysis. Results showed that PEP could induce splenocyte proliferation. In vivo experiments on Kunming mice confirmed that PEP could improve cell-mediated immunity, humoral immunity and mononuclear phagocytic system function. To illustrate the mechanism, we determined several immune-related enzymes in the thymus and spleen. The results indicated that PEP could enhance the activities of alkaline phosphatase, superoxide dismutase and lactate dehydrogenase. PEP could also increase the level of NF-κB. These results suggested that PEP exhibited potent immunomodulatory properties and could be used as a novel potential immunostimulant in food and pharmaceutical industries.
本研究旨在评估孔石莼多糖（PEP）对细胞介导免疫、体液免疫和单核吞噬细胞系统功能的影响，以评估这些大分子的免疫调节潜力。通过生化测定和 Western blot 分析验证了相关的免疫机制。结果表明，PEP 可诱导脾细胞增殖。昆明小鼠的体内实验证实，PEP 可改善细胞介导免疫、体液免疫和单核吞噬细胞系统功能。为了阐明其机制，我们测定了胸腺和脾脏中的几种与免疫相关的酶。结果表明，PEP 可增强碱性磷酸酶、超氧化物歧化酶和乳酸脱氢酶的活性。PEP 还可以增加 NF-κB 的水平。这些结果表明 PEP 具有较强的免疫调节特性，可作为食品和制药行业新型有潜力的免疫刺激剂。
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