Department of Biomedical Science, Catholic University of Daegu, Gyeongsan, Gyeongbuk, 712-702, Korea.
Int J Biol Macromol. 2011 Dec 1;49(5):1051-8. doi: 10.1016/j.ijbiomac.2011.08.032. Epub 2011 Sep 2.
Water-soluble sulfated polysaccharides extracted from Enteromorpha prolifera and fractionated using ion-exchange chromatography (crude, F(1), F(2) and F(3) fractions) were investigated to determine their in vitro and in vivo immunomodulatory activities. The sulfated polysaccharides, especially the F(1) and F(2) fractions, stimulated a macrophage cell line, Raw 264.7, inducing considerable nitric oxide (NO) and various cytokine production via up-regulated mRNA expression. The in vivo experiment results show that the sulfated polysaccharides (the crude and F(2) fractions) significantly increased Con A-induced splenocyte proliferation, revealing their potential comitogenic activity. In addition, IFN-γ and IL-2 secretions were considerably increased by the F(2) fraction without altering the release of IL-4 and IL-5. This implies that the F(2) fraction can activate T cells by up-regulating Th-1 response and that Th-1 cells might be the main target cells of the F(2) fraction. These in vitro and in vivo results suggest that the sulfated polysaccharides are strong immunostimulators.
从石莼中提取的水溶性硫酸化多糖，经离子交换层析分离（粗提物、F(1)、F(2)和 F(3)级分），研究其体外和体内免疫调节活性。硫酸化多糖，特别是 F(1)和 F(2)级分，刺激巨噬细胞系 Raw 264.7，通过上调 mRNA 表达诱导大量一氧化氮 (NO) 和各种细胞因子的产生。体内实验结果表明，硫酸化多糖（粗提物和 F(2)级分）显著增加 ConA 诱导的脾细胞增殖，显示其潜在的协同刺激活性。此外，F(2)级分可显著增加 IFN-γ和 IL-2 的分泌，而不改变 IL-4 和 IL-5 的释放。这表明 F(2)级分可以通过上调 Th1 反应激活 T 细胞，Th1 细胞可能是 F(2)级分的主要靶细胞。这些体外和体内结果表明，硫酸化多糖是强免疫刺激剂。
