Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Lab Chip. 2014 Feb 7;14(3):569-576. doi: 10.1039/c3lc51022k.
Cell size, measured as either volume or mass, is a fundamental indicator of cell state. Far more tightly regulated than size is density, the ratio between mass and volume, which can be used to distinguish between cell populations even when volume and mass appear to remain constant. Here we expand upon a previous method for measuring cell density involving a suspended microchannel resonator (SMR). We introduce a new device, the dual SMR, as a high-precision instrument for measuring single-cell mass, volume, and density using two resonators connected by a serpentine fluidic channel. The dual SMR designs considered herein demonstrate the critical role of channel geometry in ensuring proper mixing and damping of pressure fluctuations in microfluidic systems designed for precision measurement. We use the dual SMR to compare the physical properties of two well-known cancer cell lines: human lung cancer cell H1650 and mouse lymphoblastic leukemia cell line L1210.
细胞大小（以体积或质量来衡量）是细胞状态的基本指标。与大小相比，密度受到更为严格的调控，它是质量与体积的比值，可以用来区分细胞群体，即使在体积和质量似乎保持不变的情况下也是如此。在这里，我们扩展了之前使用悬浮微通道谐振器（SMR）测量细胞密度的方法。我们引入了一种新的设备，即双 SMR，作为一种高精度仪器，用于使用两个通过蛇形流道连接的谐振器来测量单细胞的质量、体积和密度。本文中考虑的双 SMR 设计证明了通道几何形状在确保微流控系统中压力波动的适当混合和阻尼方面的关键作用，该系统旨在进行精确测量。我们使用双 SMR 比较了两种著名的癌细胞系的物理特性：人肺癌细胞 H1650 和小鼠淋巴母细胞瘤系 L1210。
