Miller H, Miller B, Ryan S J
Invest Ophthalmol Vis Sci. 1986 Nov;27(11):1644-52.
The extravascular milieu around laser-induced experimental subretinal neovascularization (SRN) was studied during the evolution of the neovascular membrane from its early leaky stage to its late involuted stage. When the first signs of visible leakage appeared on angiography, newly formed vessels were spread in the subretinal space around the break in Bruch's membrane, fluid was accumulating in the subretinal space, and retinal pigment epithelial (RPE) cells were proliferating in a papillary pattern around the newly formed vessels; the RPE proliferation began with the undamaged cells at the edges of the laser injury. With further maturation, the RPE continued to envelope the subretinal vessels. This RPE proliferation was associated with the disappearance of fluid between the enveloped vessels and the sensory retina, and the gradual cessation of fluorescein leakage during angiography. At the end of the involution process, when the neovascular membrane no longer demonstrated any leakage, the subretinal vessels were found to be tightly enveloped by RPE cells, and no fluid separated them from the sensory retina. The authors' results suggest that involution of the neovascular membrane with maturation, as demonstrated by the cessation of visible fluorescein leakage, is the result of RPE proliferation that tightly envelopes the newly formed vessels and probably resorbs the previously accumulated subretinal fluid, as well as preventing its further accumulation in the subretinal space.
在激光诱导的实验性视网膜下新生血管形成（SRN）的新生血管膜从早期渗漏阶段发展到晚期退化阶段的过程中，对其血管外环境进行了研究。当血管造影上出现可见渗漏的最初迹象时，新生血管散布在 Bruch 膜破裂处周围的视网膜下间隙，视网膜下间隙有液体积聚，视网膜色素上皮（RPE）细胞围绕新生血管呈乳头状增殖；RPE 增殖始于激光损伤边缘未受损的细胞。随着进一步成熟，RPE 继续包裹视网膜下血管。这种 RPE 增殖与被包裹血管和感觉视网膜之间液体的消失以及血管造影期间荧光素渗漏的逐渐停止有关。在退化过程结束时，当新生血管膜不再显示任何渗漏时，发现视网膜下血管被 RPE 细胞紧密包裹，并且没有液体将它们与感觉视网膜分隔开。作者的结果表明，如可见荧光素渗漏停止所证明的那样，新生血管膜随着成熟而退化，是 RPE 增殖的结果，RPE 增殖紧密包裹新形成的血管，并可能吸收先前积聚的视网膜下液体，以及防止其在视网膜下间隙进一步积聚。
