Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
Carbohydr Polym. 2014 Jan 30;101:457-63. doi: 10.1016/j.carbpol.2013.09.082. Epub 2013 Sep 30.
The bark of Parkia biglobosa is used in traditional medicine to cure a wide range of illnesses. Polysaccharides were extracted from the bark with 50% ethanol-water, 50°C and 100°C water, and seven active fractions obtained by anion exchange chromatography and gel filtration. The complement fixation and macrophage stimulating activities of the different fractions were determined. The acidic fractions PBEII-I and PBEII-IV were the most active in the complement fixation assay, but the other fractions were also potent compared to the positive control BPII from Biophytum petersianum. Fractions PBEII-I and PBEII-IV were also the most potent fractions in stimulating macrophages to release nitric oxide. Structural studies showed that PBEII-I and PBEII-IV were pectic type polysaccharides, containing arabinogalactan type II structures. The observed differences in biological activities among the seven purified polysaccharide sub-fractions are probably due to differences in monosaccharide compositions, linkage types and molecular sizes.
百香果皮在传统医学中被用于治疗多种疾病。多糖用 50%乙醇-水、50°C 和 100°C 水从树皮中提取，经阴离子交换层析和凝胶过滤得到七个活性级分。测定了不同级分的补体固定和巨噬细胞刺激活性。酸性级分 PBEII-I 和 PBEII-IV 在补体固定测定中最活跃，但与阳性对照 Biophytum petersianum 的 BPII 相比，其他级分也很有效。级分 PBEII-I 和 PBEII-IV 也是刺激巨噬细胞释放一氧化氮的最有效级分。结构研究表明，PBEII-I 和 PBEII-IV 是果胶型多糖，含有阿拉伯半乳聚糖 II 型结构。七种纯化多糖亚级分之间观察到的生物活性差异可能是由于单糖组成、键合类型和分子量的差异所致。
