细胞培养过程中热塑性微流控器件中的氧含量。
Oxygen levels in thermoplastic microfluidic devices during cell culture.
机构信息
Singapore MIT Alliance for Research and Technology, BioSystems and Micromechanics, 1 CREATE Way, #04-13/14 Enterprise Wing, Singapore 138602, Singapore.
出版信息
Lab Chip. 2014 Feb 7;14(3):459-62. doi: 10.1039/c3lc51160j.
Abstract
We developed a computational model to predict oxygen levels in microfluidic plastic devices during cell culture. This model is based on experimental evaluation of oxygen levels. Conditions are determined that provide adequate oxygen supply to two cell types, hepatocytes and endothelial cells, either by diffusion through the plastic device, or by supplying a low flow rate of medium.
摘要
我们开发了一种计算模型，用于预测细胞培养过程中微流控塑料设备中的氧水平。该模型基于氧水平的实验评估。确定了通过塑料设备扩散或提供低流速培养基来为两种细胞类型（肝细胞和内皮细胞）提供足够氧气供应的条件。
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Lab Chip. 2013 Apr 21;13(8):1586-92. doi: 10.1039/c3lc41366g.
2
Construction of oxygen and chemical concentration gradients in a single microfluidic device for studying tumor cell-drug interactions in a dynamic hypoxia microenvironment.在单个微流控装置中构建氧气和化学浓度梯度，以研究动态低氧微环境中肿瘤细胞与药物的相互作用。
Lab Chip. 2013 Feb 21;13(4):695-705. doi: 10.1039/c2lc40661f.
3
A novel microfluidic platform for high-resolution imaging of a three-dimensional cell culture under a controlled hypoxic environment.一种新型微流控平台，可在受控低氧环境下对三维细胞培养进行高分辨率成像。
Lab Chip. 2012 Nov 21;12(22):4855-63. doi: 10.1039/c2lc40306d.
4
One step high quality poly(dimethylsiloxane)-hydrocarbon plastics bonding.一步法高品质聚二甲基硅氧烷-碳氢化合物塑料黏合。
Biomicrofluidics. 2012 Mar;6(1):16507-165078. doi: 10.1063/1.3694251. Epub 2012 Mar 14.
5
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Cell Death Dis. 2011 Dec 22;2(12):e246. doi: 10.1038/cddis.2011.128.
6
Design and development of microbioreactors for long-term cell culture in controlled oxygen microenvironments.用于在受控氧微环境中长期细胞培养的微生物反应器的设计和开发。
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7
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