Kareparamban J A, Nikam P H, Jadhav A P, Kadam V J
Department of Quality Assurance, Bharati Vidyapeeth's College of Pharmacy, Belapur, Navi Mumbai-400 614, India.
Indian J Pharm Sci. 2013 Jul;75(4):493-5. doi: 10.4103/0250-474X.119826.
A high-performance liquid chromatography method was developed for the estimation of ferulic acid from asafoetida and a polyherbal preparation. The separation was carried out on HiQSil ODS C-18 column with a mobile phase of acetonitrile: 10% acetic acid (20:80 v/v). The developed method was validated as per International Conference of Harmonization guidelines for various parameters such as accuracy, precision, linearity, limit of detection, limit of quantification and specificity; and found to be reliable. Linear regression analysis showed a good corelation between peak area and concentration with a corelation coefficient r(2)=0.996 in the range 200-7000 ng/ml. The developed method can be utilized for standardization of herbal formulation comprising asafoetida.
建立了一种高效液相色谱法，用于测定阿魏和一种多草药制剂中的阿魏酸。分离在HiQSil ODS C-18柱上进行，流动相为乙腈：10%乙酸（20:80 v/v）。按照国际协调会议指南对所建立的方法进行了准确度、精密度、线性、检测限、定量限和专属性等各项参数的验证，结果表明该方法可靠。线性回归分析表明，在200-7000 ng/ml范围内，峰面积与浓度之间具有良好的相关性，相关系数r(2)=0.996。所建立的方法可用于含阿魏的草药制剂的标准化。
