一种用于测定阿魏和多草药制剂中阿魏酸含量的高效液相色谱验证方法。

A Validated High-performance Liquid Chromatograhy Method for Estimation of Ferulic Acid in Asafoetida and Polyherbal Preparation.

作者信息

Kareparamban J A, Nikam P H, Jadhav A P, Kadam V J

机构信息

Department of Quality Assurance, Bharati Vidyapeeth's College of Pharmacy, Belapur, Navi Mumbai-400 614, India.

出版信息

Indian J Pharm Sci. 2013 Jul;75(4):493-5. doi: 10.4103/0250-474X.119826.

DOI:10.4103/0250-474X.119826

PMID:24302807

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3831734/

Abstract

A high-performance liquid chromatography method was developed for the estimation of ferulic acid from asafoetida and a polyherbal preparation. The separation was carried out on HiQSil ODS C-18 column with a mobile phase of acetonitrile: 10% acetic acid (20:80 v/v). The developed method was validated as per International Conference of Harmonization guidelines for various parameters such as accuracy, precision, linearity, limit of detection, limit of quantification and specificity; and found to be reliable. Linear regression analysis showed a good corelation between peak area and concentration with a corelation coefficient r(2)=0.996 in the range 200-7000 ng/ml. The developed method can be utilized for standardization of herbal formulation comprising asafoetida.

摘要

建立了一种高效液相色谱法，用于测定阿魏和一种多草药制剂中的阿魏酸。分离在HiQSil ODS C-18柱上进行，流动相为乙腈：10%乙酸（20:80 v/v）。按照国际协调会议指南对所建立的方法进行了准确度、精密度、线性、检测限、定量限和专属性等各项参数的验证，结果表明该方法可靠。线性回归分析表明，在200-7000 ng/ml范围内，峰面积与浓度之间具有良好的相关性，相关系数r(2)=0.996。所建立的方法可用于含阿魏的草药制剂的标准化。