Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Eur Rev Med Pharmacol Sci. 2011 Feb;15(2):157-64.
The Ferula genus (Umbelliferae) is a rich source of gum-resin and is much utilized in folklore medicine. This study is designed to examine antioxidant and antihaemolytic activities of Ferula foetida regel flower, stem and leaf extracts.
1,1-Diphenyl-2-picryl hydrazyl radical (DPPH), nitric oxide and H2O2 scavenging activities, Fe2+ chelating ability, reducing power and hemoglobin-induced linoleic acid peroxidation were used to evaluate antioxidant activities. Antihaemolytic activity was evaluated by H2O2 induced hemolysis in rat erythrocyte. Total phenolic compounds were determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve.
The leaf aqueous-ethanol extract showed the highest activity in DPPH radical scavenging activity. All extracts showed weak nitric oxide scavenging activity. The stem extract had better activity in nitric oxide scavenging model than the other extracts (IC50 = 896.9 +/- 21.9 microg ml(-1)), but it was not comparable to quercetin (p < 0.001). The leaf extract exhibited better H2O2 scavenging and Fe2+ chelating activity than the other parts. The extracts exhibited good antioxidant activity in linoleic acid peroxidation test but were not comparable to vitamin C (p < 0.001). Extracts showed weak reducing power activity. The stem extract showed better antihaemolytic activity than the flower and leaf. The flower extract had higher phenolic contents. The extracts exhibited different levels of antioxidant and antihaemolytic activities in all tested models.
This study showed remarkable antioxidant and antihemolytic activities in Feruia foetida. Biological effects may be attributed to the presence of phenols and flavonoids in the extract. It is very promising for further biochemical experiments.
阿魏属（伞形科）是一种丰富的树脂来源，在民间医学中被广泛应用。本研究旨在研究阿魏 foetida regel 花、茎和叶提取物的抗氧化和抗溶血活性。
1,1-二苯基-2-苦基肼基（DPPH）自由基、一氧化氮和 H2O2 清除活性、Fe2+螯合能力、还原能力和血红蛋白诱导的亚油酸过氧化用于评估抗氧化活性。通过 H2O2 诱导大鼠红细胞溶血来评估抗溶血活性。总酚类化合物用没食子酸当量表示，总黄酮含量用槲皮素当量根据校准曲线计算。
叶水-乙醇提取物在 DPPH 自由基清除活性中表现出最高的活性。所有提取物对一氧化氮清除活性均较弱。与其他提取物相比，茎提取物在一氧化氮清除模型中具有更好的活性（IC50 = 896.9 +/- 21.9 microg ml(-1)），但与槲皮素相比并不相当（p < 0.001）。叶提取物表现出更好的 H2O2 清除和 Fe2+螯合活性。提取物在亚油酸过氧化试验中表现出良好的抗氧化活性，但与维生素 C 相比不相当（p < 0.001）。提取物表现出较弱的还原能力活性。茎提取物表现出比花和叶更好的抗溶血活性。花提取物具有较高的酚含量。提取物在所有测试模型中表现出不同程度的抗氧化和抗溶血活性。
本研究表明阿魏 foetida 具有显著的抗氧化和抗溶血活性。生物效应可能归因于提取物中存在酚类和类黄酮。这对于进一步的生化实验非常有前途。
