阿魏中抗甲型流感病毒（H1N1）的抗病毒及细胞毒性药物

Influenza A (H(1)N(1)) Antiviral and Cytotoxic Agents from Ferula assa-foetida.

作者信息

Lee Chia-Lin, Chiang Lien-Chai, Cheng Li-Hung, Liaw Chih-Chuang, Abd El-Razek Mohamed H, Chang Fang-Rong, Wu Yang-Chang

机构信息

Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China.

出版信息

J Nat Prod. 2009 Sep;72(9):1568-72. doi: 10.1021/np900158f.

DOI:10.1021/np900158f

PMID:19691312

Abstract

Two new sesquiterpene coumarins, designated 5'-acetoxy-8'-hydroxyumbelliprenin (1) and 10'R-acetoxy-11'-hydroxyumbelliprenin (2), and a new diterpene, 15-hydroxy-6-en-dehydroabietic acid (3), along with 27 known compounds, were isolated from a CHCl(3)-soluble extract of Ferula assa-foetida through bioassay-guided fractionation. The structures of the new metabolites 1-3 were identified by spectroscopic data interpretation and by the Mosher ester method. Compounds 4 and 6-13 showed greater potency against influenza A virus (H(1)N(1)) (IC(50) 0.26-0.86 microg/mL) than amantadine (IC(50) 0.92 microg/mL), and 11 exhibited the best potency (IC(50) 0.51, 2.6, and 3.4 microg/mL) of these compounds against the HepG2, Hep3B, and MCF-7 cancer cell lines, respectively.

摘要

通过生物活性导向分离，从阿魏（Ferula assa-foetida）的三氯甲烷可溶提取物中分离出两种新的倍半萜香豆素，分别命名为5'-乙酰氧基-8'-羟基伞形阿魏素（1）和10'R-乙酰氧基-11'-羟基伞形阿魏素（2），以及一种新的二萜，15-羟基-6-烯-脱氢枞酸（3），同时还分离出27种已知化合物。通过光谱数据解析和Mosher酯法鉴定了新代谢产物1-3的结构。化合物4和6-13对甲型流感病毒（H(1)N(1)）的活性（IC(50) 0.26 - 0.86 μg/mL）比金刚烷胺（IC(50) 0.92 μg/mL）更强，并且化合物11对HepG2、Hep3B和MCF-7癌细胞系分别表现出最佳活性（IC(50)分别为0.51、2.6和3.4 μg/mL）。