Tan Dun-Xian, Hardeland R, Manchester L C, Galano A, Reiter R J
Department of Cellular and Structural Biology, The University of Texas, Health Science Center, San Antonio, Texas, 78229, USA.
Curr Med Chem. 2014;21(13):1557-65. doi: 10.2174/0929867321666131129113146.
Cyclic 3-hydroxymelatonin (C3HOM) is an immediate product of melatonin's interaction with reactive oxygen species. Its presence has been detected in mice, rats and humans. In the current study, the antioxidant capacity and reducing power of this molecule have been systematically studied. C3HOM is found to be a more potent antioxidant than melatonin or vitamin C in terms of its ability to scavenge the hydroxyl radical (HO.) and to recover oxidized horseradish peroxidase to its ground state. The antioxidative mechanism of C3HOM is similar to that of the classic antioxidant, vitamin C, rather than to its precursor melatonin. C3HOM effectively prevents the oxidative degradation of cytochrome C induced by hydrogen peroxide (H2O2). It is speculated that some antioxidative activities of melatonin may be mediated by its metabolite, C3HOM. C3HOM prevents mitochondrial cytochrome C injury and, thus, it is likely to inhibit cellular apoptosis induced by the release of oxidized cytochrome C from mitochondria.
环3-羟基褪黑素（C3HOM）是褪黑素与活性氧相互作用的直接产物。已在小鼠、大鼠和人类中检测到其存在。在当前研究中，对该分子的抗氧化能力和还原能力进行了系统研究。就清除羟基自由基（HO·）以及将氧化的辣根过氧化物酶恢复到基态的能力而言，发现C3HOM是比褪黑素或维生素C更有效的抗氧化剂。C3HOM的抗氧化机制与经典抗氧化剂维生素C相似，而非与其前体褪黑素相似。C3HOM有效地防止了过氧化氢（H2O2）诱导的细胞色素C的氧化降解。据推测，褪黑素的一些抗氧化活性可能由其代谢产物C3HOM介导。C3HOM可防止线粒体细胞色素C损伤，因此，它可能抑制因线粒体释放氧化型细胞色素C而诱导的细胞凋亡。
