Rajkumar K, Ramya R, Nandhini G, Rajashree P, Ramesh Kumar A, Nirmala Anandan S
Department of Oral and Maxillofacial pathology, SRM Dental College, SRM University, Chennai, India.
Oral Dis. 2015 Jan;21(1):90-6. doi: 10.1111/odi.12216. Epub 2013 Dec 30.
CYFRA 21-1, a constituent of the intermediate filament proteins of epithelial cells, is known to be increased in many cancers. This study was designed to estimate the levels of salivary and serum CYFRA 21-1 in patients with oral precancer and oral squamous cell carcinoma (OSCC) and compare them with healthy controls.
Each group comprised of 100 subjects. Saliva and blood samples were collected from patients with OSCC, premalignant subjects, and normal healthy subjects. Serum and salivary CYFRA 21-1 levels were measured by enzyme-linked immunosorbent assay. Appropriate statistical tests were employed to assess diagnostic potency of CYFRA 21-1.
We found a significant increase in CYFRA 21-1 level in OSCC compared with PML and healthy subjects. Salivary CYFRA 21-1 levels in OSCC was threefold higher when compared to serum levels. PML group showed increased salivary CYFRA 21-1 when compared to control subjects, but it was significantly lower compared with OSCC. Receiver operator characteristic curve analysis showed salivary CYFRA 21-1 to have superior sensitivity in detecting OSCC compared with serum CYFRA 21-1.
The outcome of this study suggests that salivary CYFRA 21-1 can be utilized as a biomarker in early detection of oral cancer.
细胞角蛋白19片段（CYFRA 21-1）是上皮细胞中间丝蛋白的一个组成部分，已知在多种癌症中会升高。本研究旨在评估口腔癌前病变和口腔鳞状细胞癌（OSCC）患者唾液和血清中CYFRA 21-1的水平，并与健康对照进行比较。
每组包括100名受试者。收集OSCC患者、癌前病变受试者和正常健康受试者的唾液和血液样本。采用酶联免疫吸附测定法测量血清和唾液中CYFRA 21-1的水平。采用适当的统计学检验来评估CYFRA 21-1的诊断效能。
我们发现与癌前病变和健康受试者相比，OSCC患者的CYFRA 21-1水平显著升高。OSCC患者的唾液CYFRA 21-1水平是血清水平的三倍。与对照受试者相比，癌前病变组的唾液CYFRA 21-1升高，但与OSCC相比显著较低。受试者工作特征曲线分析显示，与血清CYFRA 21-1相比，唾液CYFRA 21-1在检测OSCC方面具有更高的敏感性。
本研究结果表明，唾液CYFRA 21-1可作为口腔癌早期检测的生物标志物。
