Bel'skaya Lyudmila V, Sarf Elena A, Loginova Alexandra I, Vyushkov Dmitry M, Choi En Djun
Biochemistry Research Laboratory, Omsk State Pedagogical University, 14, Tukhachevsky Str., 644099 Omsk, Russia.
Department of Biochemistry, Omsk State Medical University, 12, Lenina Str., 644099 Omsk, Russia.
Curr Issues Mol Biol. 2023 Jun 10;45(6):5084-5098. doi: 10.3390/cimb45060323.
The aim of the study was to determine the content of tumor markers for breast, lung and ovarian cancer in saliva, as well as for benign diseases of the corresponding organs and in the control group, and to evaluate their diagnostic significance. Strictly before the start of treatment, saliva samples were obtained and the concentrations of tumor markers (AFP, NSE, HE4, CA15-3, CA72-4, CA125 and CEA) were determined using an enzyme immunoassay (ELISA). CA125 and HE4 were simultaneously determined to be in the blood serum of patients with ovarian cancer. The concentrations of salivary CEA, NSE, CA15-3, CA72-4 and CA125 of the control group were significantly lower than in oncological diseases; however, these tumor markers also increased in saliva with benign diseases. The content of tumor markers depends on the stage of cancer, and the presence of lymph node metastasis; however, the identified patterns are statistically unreliable. The determination of HE4 and AFP in saliva was not informative. In general, the area of potential use of tumor markers in saliva is extremely narrow. Thus, CEA may be diagnostic for breast and lung cancer, but not for ovarian cancer. CA72-4 is most informative for ovarian mucinous carcinoma. None of the markers showed significant differences between malignant and non-malignant pathologies.
该研究的目的是测定唾液中乳腺癌、肺癌和卵巢癌的肿瘤标志物含量，以及相应器官良性疾病患者和对照组的肿瘤标志物含量，并评估其诊断意义。在严格治疗开始前采集唾液样本，采用酶联免疫吸附测定法（ELISA）测定肿瘤标志物（甲胎蛋白、神经元特异性烯醇化酶、人附睾蛋白4、癌抗原15-3、癌抗原72-4、癌抗原125和癌胚抗原）的浓度。同时测定卵巢癌患者血清中的癌抗原125和人附睾蛋白4。对照组唾液中癌胚抗原、神经元特异性烯醇化酶、癌抗原15-3、癌抗原72-4和癌抗原125的浓度显著低于肿瘤疾病患者；然而，这些肿瘤标志物在良性疾病患者的唾液中也有所升高。肿瘤标志物的含量取决于癌症分期和淋巴结转移情况；然而，所确定的模式在统计学上并不可靠。唾液中人附睾蛋白4和甲胎蛋白的测定并无诊断价值。总体而言，唾液中肿瘤标志物的潜在应用范围极为狭窄。因此，癌胚抗原可能对乳腺癌和肺癌有诊断价值，但对卵巢癌无诊断价值。癌抗原72-4对卵巢黏液性癌最具诊断价值。没有一种标志物在恶性和非恶性病变之间显示出显著差异。
