Kitagawa H, Takeda F, Kohei H
Arzneimittelforschung. 1986 Aug;36(8):1240-4.
In the present study Corne's technique that has been commonly used to quantify the gastric mucus in situ using the dye, Alcian blue, has been improved. Furthermore, the effects of antiulcerogenic agents on the decrease in gastric mucus gel during water-immersion stress were also studied. In order to increase the extraction of dye-recovery, we used 30% dioctyl sodium sulfosuccinate (docusate sodium, DSS) instead of 0.5 mol/l MgCl2 used in Corne's technique. The control values of the dye-recovery extracted with DSS were about 5 times higher than those with MgCl2. Indomethacin (20 mg/kg s.c.) failed to influence dye-recovery with DSS and MgCl2, while reserpine (5 mg/kg i.p.) significantly decreased the dye-recovery in both extractions. Water-immersion stress for 2 h decreased the dye-recovery with DSS but the dye-recovery with MgCl2 decreased only 15 min after water-immersion. These results suggest that extraction with DSS reflects the mucus gel secretion, and that dye-recovery with DSS is more sensitive to alteration of mucus gel than that with MgCl2. Bombesin (10 micrograms/kg), secretin (100 U/kg) and mild irritants increased the dye-recovery with DSS. Cimetidine remarkably reduced the mucus secretion and failed to improve the decrease in mucus during water-immersion stress. Pirenzepine and atropine dose-dependently prevented the decrease in the dye-recovery during water-immersion stress.
在本研究中，常用于原位定量胃黏液的科恩技术（使用阿尔新蓝染料）得到了改进。此外，还研究了抗溃疡药物对水浸应激期间胃黏液凝胶减少的影响。为了提高染料回收率，我们使用30%磺基琥珀酸二辛酯钠（多库酯钠，DSS）替代科恩技术中使用的0.5 mol/l氯化镁。用DSS提取的染料回收率对照值比用氯化镁提取的高约5倍。吲哚美辛（20 mg/kg，皮下注射）对用DSS和氯化镁提取的染料回收率均无影响，而利血平（5 mg/kg，腹腔注射）在两种提取方法中均显著降低了染料回收率。水浸应激2小时会降低用DSS提取的染料回收率，但用氯化镁提取的染料回收率仅在水浸后15分钟下降。这些结果表明，用DSS提取反映了黏液凝胶的分泌，并且用DSS提取的染料回收率比用氯化镁提取的对黏液凝胶的变化更敏感。蛙皮素（10微克/千克）、促胰液素（100单位/千克）和轻度刺激剂可提高用DSS提取的染料回收率。西咪替丁显著减少黏液分泌，且未能改善水浸应激期间黏液的减少。哌仑西平和阿托品在水浸应激期间剂量依赖性地阻止染料回收率下降。
