Institut für Botanik, Universität Würzburg, Lehrstuhl Botanik 1, Mittlerer Dallenbergweg 64, D-8700, Würzburg, Federal Republic of Germany.
Planta. 1986 Mar;167(3):376-81. doi: 10.1007/BF00391342.
Spinach (Spinacia oleracea L.) and barley (Hordeum vulgare L.) were grown in hydroponic culture with varying levels of orthophosphate (Pi). When leaves were fed with 20 mmol·l(-1) Pi at low CO2 concentrations, a temporary increase of CO2 uptake was observed in Pi-deficient leaves but not in those from plants grown at 1 mmol·l(-1) Pi. At high concentrations of CO2 (at 21% or 2% O2) the Pi-induced stimulation of CO2 uptake was pronounced in the Pi-deficient leaves. The contents of phosphorylated metabolites in the leaves decreased as a result of Pi deficiency but were restored by Pi feeding. These results demonstrate that there is an appreciable capacity for rapid Pi uptake by leaf mesophyll cells and show that the effects of long-term phosphate deficiency on photosynthesis may be reversed (at least temporarily) within minutes by feeding with Pi.
菠菜（Spinacia oleracea L.）和大麦（Hordeum vulgare L.）在水培条件下，用不同浓度的正磷酸盐（Pi）进行培养。当叶片在低 CO2 浓度下用 20mmol·l(-1) Pi 进行喂养时，在 Pi 缺乏的叶片中观察到 CO2 摄取的暂时增加，但在来自生长在 1mmol·l(-1) Pi 条件下的叶片中未观察到。在高浓度的 CO2（21%或 2% O2）下，Pi 缺乏的叶片中，Pi 诱导的 CO2 摄取刺激作用明显。由于 Pi 缺乏，叶片中磷酸化代谢物的含量减少，但通过 Pi 喂养得到恢复。这些结果表明，叶片的叶肉细胞具有相当大的快速吸收 Pi 的能力，并且表明长期缺磷对光合作用的影响可以在几分钟内通过 Pi 喂养得到逆转（至少暂时逆转）。
