Department of Anesthesiology, Herlev Hospital, University of Copenhagen, Herlev Ringvej 75, 2730, Herlev, Denmark,
J Clin Monit Comput. 2014 Aug;28(4):351-5. doi: 10.1007/s10877-013-9539-1. Epub 2013 Dec 5.
Intense neuromuscular blockade (NMB) measured by post tetanic count (PTC) was monitored, reversed and verified in this pig model. In a cross-over assessor blinded design six pigs were randomized to either no NMB followed by intense NMB, or intense NMB followed by no NMB. Neuromuscular measurements were performed with acceleromyography [train-of-four (TOF) Watch SX]. In all pigs, the response to TOF nerve stimulation was stable and intense NMB (PTC 0-1) was established with rocuronium 3 mg/kg. For reversal, the pigs received sugammadex 20-35 mg/kg and returned to TOF-ratio above 0.90 within 2.15 min after injection. We established a pig model for monitoring intense NMB with surface stimulation electrodes and acceleromyography. We verified total relaxation of the diaphragm and the abdominal muscles at the PTC 0-1 by suction test and with surface electromyography. This pig model is suitable for studies with experimental abdominal surgery with monitoring of intense NMB, and where relaxation of the diaphragm and the abdominal muscles are required.
本研究通过强直刺激后计数（PTC）监测、逆转和验证了猪模型中的强神经肌肉阻滞（NMB）。在交叉评估者盲法设计中，将 6 头猪随机分为无 NMB 后出现强 NMB 组，或强 NMB 后无 NMB 组。肌电图[四串刺激（TOF）Watch SX]用于进行神经肌肉测量。在所有猪中，TOF 神经刺激的反应稳定，罗库溴铵 3mg/kg 可实现强 NMB（PTC 0-1）。逆转时，猪接受琥珀酸舒更葡糖钠 20-35mg/kg，注射后 2.15 分钟内 TOF 比值恢复至 0.90 以上。我们建立了一种通过表面刺激电极和肌电图监测强 NMB 的猪模型。我们通过负压试验和表面肌电图验证了 PTC 0-1 时膈肌和腹肌的完全松弛。该猪模型适用于监测强 NMB 的实验性腹部手术研究，以及需要膈肌和腹肌松弛的研究。
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