Wiedermann C J, Sertl K, Pert C B
Blood. 1986 Dec;68(6):1398-401.
The interaction of substance P with intact lymphatic tissue was quantified and autoradiographically visualized, using slide-mounted tissue sections of rat spleen. Radiolabeled substance P binds rapidly to an apparently single class of noninteracting high affinity sites (Kd = 2.4 nmol/L; Bmax = 9.4 fmol/mg protein). The ligand selectivity pattern suggests that substance P binding sites are similar to substance P receptors found in other tissues, including the brain, T lymphocytes, and macrophages. Substance P receptors are highly concentrated in the antigen-trapping spleen marginal zone, with low densities being found in the red pulp. No specific binding of radiolabel to T cell-dependent immunologic domains of the spleen is seen. The distribution of substance P receptors suggests that substance P is probably involved in the control of sensory functions of the immune system.
利用大鼠脾脏的载玻片组织切片，对P物质与完整淋巴组织的相互作用进行了定量，并通过放射自显影进行了可视化观察。放射性标记的P物质迅速与一类明显单一的非相互作用高亲和力位点结合（解离常数Kd = 2.4 nmol/L；最大结合容量Bmax = 9.4 fmol/mg蛋白质）。配体选择性模式表明，P物质结合位点与在其他组织（包括脑、T淋巴细胞和巨噬细胞）中发现的P物质受体相似。P物质受体高度集中在捕获抗原的脾脏边缘区，在红髓中的密度较低。未观察到放射性标记物与脾脏T细胞依赖性免疫区域的特异性结合。P物质受体的分布表明，P物质可能参与免疫系统感觉功能的控制。
