Department of Biology, University of Victoria, V8W 2Y2, Victoria, British Columbia, Canada.
J Chem Ecol. 1986 Apr;12(4):915-32. doi: 10.1007/BF01020261.
The application of an ultrasensitive photobiological assay which detects photosensitizing furocoumarins with sensitivities as high as 1 × 10(-11) g is discussed in relation to these molecules as phytoalexins. Examples of the utilization of this technique, verified by both HPLC and TLC, are the analyses of healthy and diseased celery and carrots, dry seeds, plant extracts and oils, and whole plants and leaves. The usefulness of this method in following the metabolic detoxification of furocoumarins is also illustrated. The extreme sensitivity of the test has permitted the detection, for the first time, of both 5-methoxypsoralen and 8-methoxypsoralen in fresh carrot roots.
本文讨论了一种超灵敏的光生物测定法在植物抗毒素方面的应用，这种方法可以检测到灵敏度高达 1×10(-11) g 的呋喃香豆素。通过高效液相色谱法（HPLC）和薄层色谱法（TLC）验证了该技术的应用实例，包括健康和患病的芹菜和胡萝卜、干种子、植物提取物和油，以及整株植物和叶片的分析。该方法在监测呋喃香豆素的代谢解毒方面的应用也得到了说明。该测试方法的极高灵敏度首次允许在新鲜胡萝卜根中检测到 5-甲氧基补骨脂素和 8-甲氧基补骨脂素。
