John R, Henley R, Shankland D
Clin Chem. 1986 Nov;32(11):2066-9.
We evaluated a two-site enhanced luminescence immunoenzymometric assay (Amerlite; Amersham International) for alpha-fetoprotein (AFP) in maternal serum and amniotic fluid. The assay is rapid, involving two incubations totalling 4 h. The working range of the assay for serum AFP is 5.5 to 750 kilo-int. units/L (CV less than 10%), with a sensitivity of detection of 0.2 kilo-int. unit/L. The regression equation for the Amerlite assay (y) and our in-house RIA procedure (x) was y = 0.816x + 2.9 (n = 142, r = 0.96). Analytical recovery of added AFP (code 72/227) at three concentrations was 86.7%. Serum AFP concentrations were measured at 15 to 18 weeks of gestation in subjects with normal pregnancies and in subjects whose pregnancies resulted in open neural tube defects; all of the latter had serum AFP concentrations greater than 2.5 multiples of the median. We find the Amerlite system to be an efficient, reliable system for screening for open neural tube defects without use of hazardous radioactive labels.
我们评估了一种用于检测母血和羊水中甲胎蛋白（AFP）的双位点增强发光免疫酶分析法（Amerlite；Amersham国际公司）。该分析方法快速，只需进行两次温育，总共4小时。该分析方法检测血清AFP的工作范围是5.5至750千国际单位/升（变异系数小于10%），检测灵敏度为0.2千国际单位/升。Amerlite分析法（y）与我们内部的放射免疫分析法（x）的回归方程为y = 0.816x + 2.9（n = 142，r = 0.96）。添加的三种浓度的AFP（编号72/227）的分析回收率为86.7%。对正常妊娠妇女和妊娠结果为开放性神经管缺陷的妇女在妊娠15至18周时测量血清AFP浓度；所有后者的血清AFP浓度均高于中位数的2.5倍。我们发现Amerlite系统是一种高效、可靠的系统，可用于筛查开放性神经管缺陷，且无需使用有危害的放射性标记物。
