Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan, Hubei 430072, China.
Anal Chem. 2013 Dec 17;85(24):11691-4. doi: 10.1021/ac403693g. Epub 2013 Dec 5.
A paper-based microfluidic device with upconversion fluorescence assay (named as UC-μPAD) is proposed. The device is fabricated on a normal office printing sheet with a simple plotting method. Upconversion phosphors (UCPs) tagged with specific probes are spotted to the test zones on the μPAD, followed by the introduction of assay targets. Upconversion fluorescence measurements are directly conducted on the test zones after the completion of the probe-to-target reactions, without any post-treatments. The UC-μPAD features very easy fabrication and operation, simple and fast detection, low cost, and high sensitivity. UC-μPAD is a promising prospect for a clinical point-of-care test.
提出了一种基于纸张的上转换荧光分析（称为 UC-μPAD）的微流控装置。该装置采用简单的绘图方法在普通办公打印纸上制造。将标记有特定探针的上转换荧光粉（UCPs）点样到μPAD 的测试区域，然后引入检测靶标。在探针与靶标反应完成后，无需进行任何后处理，即可直接在上转换荧光测量在测试区域进行。UC-μPAD 具有制造和操作非常简单、检测简单快速、成本低、灵敏度高等特点。UC-μPAD 是临床即时检测的一个很有前景的选择。
