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吸附-解吸纳米适配体传感器：赭曲霉毒素A的荧光筛选检测法

Adsorption-desorption nano-aptasensors: fluorescent screening assays for ochratoxin A.

作者信息

Ranganathan Velu, Boisjoli Spencer, DeRosa Maria C

机构信息

Department of Chemistry, Carleton University 1125 Colonel By Drive Ottawa ON K1S 5B6 Canada

出版信息

RSC Adv. 2022 May 9;12(22):13727-13739. doi: 10.1039/d2ra00026a. eCollection 2022 May 5.

DOI:10.1039/d2ra00026a
PMID:35541430
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9081825/
Abstract

In this study, a FRET-based fluorescent aptasensor for the detection of ochratoxin A (OTA) was optimized based on the quenching efficiency of single-walled carbon nanotubes (SWCNTs) and the binding affinity of aptamers. OTA aptamers were conjugated with quantum dots and adsorbed to the surface of both acid-modified and unmodified SWCNTs. The maximum fluorescence quenching efficiency of the SWCNTs were compared. Acid-modified SWCNTs (amSWCNTs) have moderate quenching efficiency, providing an optimal sensitivity for qualitative fluorescence-enhancement biosensor assays. The binding parameters of the QD-modified OTA aptamers (1.12.2 and A08min) on the surface of amSWCNTs were compared. Based on our results, the A08min aptamer is a better candidate for OTA detection. Using the A08min aptamer, the SWCNT method had a limit of detection (LOD) of 40 nM. The amSWCNT method had a significantly lower LOD of 14 nM. Turn-on fluorescent nano-aptasensors are emerging as an effective diagnostic tool for simple detection of mycotoxins. Nanocomplexes designed for the detection of mycotoxins in solution and paper-based tests have proven to be useful.

摘要

在本研究中，基于单壁碳纳米管（SWCNTs）的猝灭效率和适配体的结合亲和力，对一种用于检测赭曲霉毒素A（OTA）的基于荧光共振能量转移（FRET）的荧光适配体传感器进行了优化。OTA适配体与量子点偶联，并吸附在酸修饰和未修饰的SWCNTs表面。比较了SWCNTs的最大荧光猝灭效率。酸修饰的SWCNTs（amSWCNTs）具有适度的猝灭效率，为定性荧光增强生物传感器检测提供了最佳灵敏度。比较了QD修饰的OTA适配体（1.12.2和A08min）在amSWCNTs表面的结合参数。根据我们的结果，A08min适配体是OTA检测的更好候选者。使用A08min适配体，SWCNT方法的检测限（LOD）为40 nM。amSWCNT方法的LOD显著更低，为14 nM。开启式荧光纳米适配体传感器正在成为一种用于简单检测霉菌毒素的有效诊断工具。设计用于检测溶液中霉菌毒素和基于纸张测试的纳米复合物已被证明是有用的。

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