Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Analyst. 2014 Apr 7;139(7):1637-43. doi: 10.1039/c3an01926h.
An inkjet-printed microfluidic paper-based analytical device (μPAD) for the detection of lactoferrin has been developed. The analyte concentration dependent fluorescence emission, caused by the sensitization of pre-deposited terbium (Tb(3+)) upon complexation with lactoferrin on the paper device, is captured using a digital camera. The dynamic response range (0.5-3 mg mL(-1)) and the limit of detection (0.30 mg mL(-1)) of the μPAD are suitable for the analysis of normal human tears and the detection of eye disorders. Finally, lactoferrin concentrations in human tear samples were analyzed by the μPADs and the assay results corresponded within 6% error to those obtained by an immunoassay (ELISA). The μPADs provide a simple, rapid and accurate method for lactoferrin detection in tear fluid. Results are obtained within 15 min of a single application of 2.5 μL of sample. To the best of our knowledge, this is the first report of a device for lactoferrin quantification relying neither on an immunoassay nor on high cost analytical instrumentation.
已经开发出一种用于检测乳铁蛋白的喷墨打印微流控纸基分析器件（μPAD）。在纸器件上通过与乳铁蛋白络合来预先沉积铽（Tb（3+）），从而导致分析物浓度依赖性的荧光发射，使用数码相机捕获该荧光发射。μPAD 的动态响应范围（0.5-3 mg mL（-1））和检测限（0.30 mg mL（-1））适用于正常人类眼泪的分析和眼部疾病的检测。最后，通过 μPAD 分析了人泪样中的乳铁蛋白浓度，并且测定结果与通过免疫测定（ELISA）获得的结果相差在 6%以内。μPAD 为在泪液中检测乳铁蛋白提供了一种简单、快速和准确的方法。在单个应用 2.5 μL 样品的 15 分钟内即可获得结果。据我们所知，这是第一个不依赖于免疫测定或高成本分析仪器的用于乳铁蛋白定量的器件的报告。
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