State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Insect Mol Biol. 2014 Apr;23(2):175-84. doi: 10.1111/imb.12074. Epub 2013 Dec 6.
RNA interference (RNAi) by introducing double-stranded RNA (dsRNA) is a powerful approach to the analysis of gene function in insects; however, RNAi responses vary dramatically in different insect species and tissues, and the underlying mechanisms remain poorly understood. The migratory locust, a destructive insect pest and a hemimetabolic insect with panoistic ovaries, is considered to be a highly susceptible species to RNAi via dsRNA injection, but its ovary appears to be completely insensitive. In the present study, we showed that dsRNA persisted only briefly in locust haemolymph. The ovariole sheath was permeable to dsRNA, but injected dsRNA was not present in the follicle cells and oocytes. The lack of dsRNA uptake into the follicle cells and oocytes is likely to be the primary factor that contributes to the ineffective RNAi response in locust ovaries. These observations provide insights into tissue-dependent variability of RNAi and help in achieving successful gene silencing in insensitive tissues.
RNA 干扰（RNAi）通过引入双链 RNA（dsRNA）是一种分析昆虫基因功能的有效方法；然而，不同昆虫物种和组织中的 RNAi 反应差异很大，其潜在机制仍知之甚少。 迁徙性蝗虫是一种具有破坏性的害虫，也是一种具有全景卵巢的半变态昆虫，被认为是通过 dsRNA 注射对 RNAi 非常敏感的物种，但它的卵巢似乎完全不敏感。 在本研究中，我们表明 dsRNA 在蝗虫血淋巴中仅短暂存在。 卵鞘对 dsRNA 具有通透性，但注射的 dsRNA 不存在于滤泡细胞和卵母细胞中。 dsRNA 不能被滤泡细胞和卵母细胞摄取，这可能是导致蝗虫卵巢中 RNAi 反应无效的主要因素。 这些观察结果为 RNAi 的组织依赖性变异性提供了深入了解，并有助于在不敏感组织中实现成功的基因沉默。
