Kawakami K, Ohta T, Nojima H, Nagano K
J Biochem. 1986 Aug;100(2):389-97. doi: 10.1093/oxfordjournals.jbchem.a121726.
Clones carrying cDNA sequences for the alpha-subunit of the Na,K-ATPase from HeLa cells have been isolated. Nucleotide sequence analysis of the cloned cDNA has revealed the primary structure of this polypeptide, which consists of 1,023 amino acids. The alpha-subunit of the human Na,K-ATPase exhibited 87% homology with its Torpedo counterpart and 98% homology with its sheep counterpart. The six putative transmembrane segments M1-M6 showed higher conservation than the total segments. Total genomic Southern hybridization indicated the existence of at most two copies, possibly only one, of the gene encoding the Na,K-ATPase alpha-subunit in the human genome.
已从人宫颈癌细胞（HeLa细胞）中分离出携带钠钾ATP酶α亚基cDNA序列的克隆。对克隆的cDNA进行核苷酸序列分析，揭示了该多肽的一级结构，它由1023个氨基酸组成。人钠钾ATP酶的α亚基与电鳐相应亚基有87%的同源性，与绵羊相应亚基有98%的同源性。六个推定的跨膜片段M1 - M6的保守性高于整个片段。全基因组Southern杂交表明，人类基因组中编码钠钾ATP酶α亚基的基因最多存在两个拷贝，也可能只有一个拷贝。
