Kawakami K, Noguchi S, Noda M, Takahashi H, Ohta T, Kawamura M, Nojima H, Nagano K, Hirose T, Inayama S
Nature. 1985;316(6030):733-6. doi: 10.1038/316733a0.
Sodium- and potassium-dependent ATPase [(Na+ + K+)ATPase], which is responsible for the active transport of Na+ and K+, is distributed universally among animal cell membranes and consists of two types of subunits, alpha and beta (refs 1-4). The larger alpha-subunit with a relative molecular mass (Mr) of 84,000-120,000 is thought to have the catalytic role. We have now cloned and sequenced DNA complementary to the Torpedo californica electroplax messenger RNA encoding the alpha-subunit of (Na+ + K+)ATPase and have deduced the complete amino-acid sequence of the polypeptide. Some structural features of the alpha-subunit molecule related to the function of this active-transport protein are discussed.
钠钾依赖ATP酶[（Na⁺+K⁺）ATP酶]负责Na⁺和K⁺的主动运输，普遍分布于动物细胞膜中，由α和β两种亚基组成（参考文献1 - 4）。相对分子质量（Mr）为84,000 - 120,000的较大α亚基被认为具有催化作用。我们现已克隆并测序了与加州电鳐电器官信使RNA互补的DNA，该信使RNA编码（Na⁺+K⁺）ATP酶的α亚基，并推导了该多肽的完整氨基酸序列。本文讨论了α亚基分子与这种主动运输蛋白功能相关的一些结构特征。
