Department of Ecology and Evolutionary Biology, University of California, Irvine, 92717, Irvine, CA.
J Chem Ecol. 1985 Jul;11(7):847-58. doi: 10.1007/BF01012073.
During isolation of two biosynthetic types of cyanogenic glycosides fromCarica papaya, weak cyanide tests were obtained from initial fractions. Upon final purification, strongly positive cyanide tests were obtained. Pretreatment of extracts to remove polyphenolics alleviated inhibition of cyanogenesis, which led us to suspect that tannins were inhibitory agents. Qualitative and quantitative measures of inhibition were made using standard cyanogenic glycosides and polyphenolics. Cyanogenesis was inhibited quantitatively when condensed tannins (quebracho, wattle, and chestnut), or hydrolyzable tannin (tannic acid) were added. When tannins were precipitated from the reaction mixture, cyanide tests proceeded optimally. These results stress the need to interpret negative cyanide tests with care and indicate possible ecological synergisms between plant defensive chemicals.
在从木瓜中分离两种生物合成类型的氰苷的过程中，最初的几个部分得到了微弱的氰化物测试结果。在最终纯化后，得到了强烈的阳性氰化物测试结果。预处理提取物以去除多酚类物质，减轻了氰化作用的抑制作用，这使我们怀疑单宁是抑制因子。使用标准氰苷和多酚类物质进行了定性和定量的抑制测量。当加入缩合单宁（奎拉乔、金合欢和栗木）或可水解单宁（鞣酸）时，定量地抑制了氰化作用。当单宁从反应混合物中沉淀出来时，氰化物测试最佳进行。这些结果强调了需要谨慎解释阴性氰化物测试的必要性，并表明植物防御化学物质之间可能存在生态协同作用。
