Department of Botany, University of Durham, DH1 3LE, Durham, England.
Plant Mol Biol. 1984 Mar;3(2):91-6. doi: 10.1007/BF00040033.
The sequence of two cDNA clones coding for the whole of the β-subunit and most of the α-subunit of legumin are presented together with a considerable amount of protein sequence data to confirm the predicted amino acid sequence. A unique feature shown by these cDNAs is the presence of three 56 base pair tandem repeats in the region encoding the C terminal of the α polypeptide. The tandem repeats are also exhibited in the predicted polypeptide sequence as three 18 amino acid repeats which contain extremely high proportions of polar, mainly acidic, residues. The new sequences are compared to the previously published sequence of some shorter legumin cDNAs (Nature 295: 76-79). In the region where the sequences overlap, the previous cDNAs differ from the new ones by only a few base substitutions but most of the repeated region is not present though the sequences on either side are. The possibility that the absence of the repeats may reflect the difference between two types of legumin gene, rather than an artefact of the cloning of the cDNAs, is discussed.
本文呈现了两个 cDNA 克隆的序列，它们编码整个β亚基和大部分α亚基的豌豆球蛋白，并提供了大量的蛋白质序列数据来证实预测的氨基酸序列。这些 cDNA 显示出的一个独特特征是，在编码α多肽 C 末端的区域存在三个 56 个碱基对的串联重复。串联重复也在预测的多肽序列中表现为三个 18 个氨基酸的重复，其中包含极高比例的极性、主要是酸性的残基。新序列与先前发表的一些较短豌豆球蛋白 cDNA 的序列进行了比较（《自然》295:76-79）。在序列重叠的区域，先前的 cDNA 与新的 cDNA 只有几个碱基的差异，但重复区域的大部分都不存在，尽管两侧的序列都存在。讨论了缺失重复序列可能反映两种类型的豌豆球蛋白基因之间的差异，而不是 cDNA 克隆的人为产物的可能性。
